TY - JOUR
T1 - Mutational analysis of the Helicobacter pylori vacuolating toxin amino terminus
T2 - Identification of amino acids essential for cellular vacuolation
AU - Ye, Dan
AU - Blanke, Steven R.
PY - 2000/7
Y1 - 2000/7
N2 - The functional importance of the amino terminus of the Helicobacter pylori vacuolating cytotoxin (VacA) was investigated by analyzing the relative levels of vacuolation of HeLa cells transfected with plasmids encoding wild-type and mutant forms of the toxin. Notably, VacA's intracellular activity was found to be sensitive to small truncations and internal deletions at the toxin's amino terminus. Moreover, alanine-scanning mutagenesis revealed the first VacA point mutations (at proline 9 or glycine 14) that completely abolish the toxin's intracellular activity.
AB - The functional importance of the amino terminus of the Helicobacter pylori vacuolating cytotoxin (VacA) was investigated by analyzing the relative levels of vacuolation of HeLa cells transfected with plasmids encoding wild-type and mutant forms of the toxin. Notably, VacA's intracellular activity was found to be sensitive to small truncations and internal deletions at the toxin's amino terminus. Moreover, alanine-scanning mutagenesis revealed the first VacA point mutations (at proline 9 or glycine 14) that completely abolish the toxin's intracellular activity.
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U2 - 10.1128/IAI.68.7.4354-4357.2000
DO - 10.1128/IAI.68.7.4354-4357.2000
M3 - Article
C2 - 10858259
AN - SCOPUS:0033942982
SN - 0019-9567
VL - 68
SP - 4354
EP - 4357
JO - Infection and Immunity
JF - Infection and Immunity
IS - 7
ER -