Mutation in the cysteine bridge domain of the γ-subunit affects light regulation of the ATP synthase but not photosynthesis or growth in Arabidopsis

Guosheng Wu, Donald Richard Ort

Research output: Contribution to journalArticle

Abstract

The chloroplast ATP synthase synthesizes ATP from ADP and free phosphate coupled by the electrochemical potential across the thylakoid membrane in the light. The light-dependent regulation of ATP synthase activity is carried out in part through redox modulation of a cysteine disulfide bridge in CF 1 γ-subunit. In order to investigate the function of the redox regulatory domain and the physiological significance of redox modulation for higher plants, we designed four mutations in the redox regulatory domain of the γ-subunit to create functional mimics of the permanently reduced form of the γ-subunit. While the inability to reduce the regulatory disulfide results in lower photosynthesis and growth, unexpectedly, the results reported here show that inability to reoxidize the dithiol may not be of any direct detriment to plant photosynthetic performance or growth.

Original languageEnglish (US)
Pages (from-to)185-193
Number of pages9
JournalPhotosynthesis research
Volume97
Issue number2
DOIs
StatePublished - Aug 1 2008

Keywords

  • Chloroplast ATP synthase
  • Disulfide bond
  • Redox modulation
  • Thioredoxin

ASJC Scopus subject areas

  • Plant Science

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