Mutants of Escherichia coli defective in membrane phospholipid synthesis: mapping of sn glycerol 3 phosphate acyltransferase Km mutants

J. E. Cronan, R. M. Bell

Research output: Contribution to journalArticle

Abstract

plsB mutants of E. coli are sn glycerol 3 phosphate auxotrophs which owe their requirement to a K(m) defect in sn glycerol 3 phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. The authors located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene defined by mutants with a temperature sensitive sn glycerol 3 phosphate acyltransferase. The plsB gene was cotransduced with the dctA locus and the transduction data indicated that the clockwise gene order is asd, plsB, dctA, xyl. plsB- is recessive to plsB+ and all acyltransferase K(m) mutants tested lie very close to the plsB locus. Effective supplementation of plsB mutants was shown not to require a defective glpD gene.

Original languageEnglish (US)
Pages (from-to)227-233
Number of pages7
JournalJournal of bacteriology
Volume120
Issue number1
StatePublished - Dec 1 1974
Externally publishedYes

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Acyltransferases
Phospholipids
Escherichia coli
Membranes
Genes
Gene Order
Biosynthetic Pathways
Temperature
alpha-glycerophosphoric acid
Enzymes

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

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abstract = "plsB mutants of E. coli are sn glycerol 3 phosphate auxotrophs which owe their requirement to a K(m) defect in sn glycerol 3 phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. The authors located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene defined by mutants with a temperature sensitive sn glycerol 3 phosphate acyltransferase. The plsB gene was cotransduced with the dctA locus and the transduction data indicated that the clockwise gene order is asd, plsB, dctA, xyl. plsB- is recessive to plsB+ and all acyltransferase K(m) mutants tested lie very close to the plsB locus. Effective supplementation of plsB mutants was shown not to require a defective glpD gene.",
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AU - Bell, R. M.

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AB - plsB mutants of E. coli are sn glycerol 3 phosphate auxotrophs which owe their requirement to a K(m) defect in sn glycerol 3 phosphate acyltransferase, the first enzyme in the phospholipid biosynthetic pathway. The authors located the plsB gene at minute 69 of the E. coli genetic map, far removed from the gene defined by mutants with a temperature sensitive sn glycerol 3 phosphate acyltransferase. The plsB gene was cotransduced with the dctA locus and the transduction data indicated that the clockwise gene order is asd, plsB, dctA, xyl. plsB- is recessive to plsB+ and all acyltransferase K(m) mutants tested lie very close to the plsB locus. Effective supplementation of plsB mutants was shown not to require a defective glpD gene.

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