Mutants of Escherichia coli defective in membrane phospholipid synthesis: Mapping of the structural gene for L glycerol 3 phosphate dehydrogenase

John E Cronan, R. M. Bell

Research output: Contribution to journalArticle

Abstract

The structural gene for the biosynthetic L glycerol 3 phosphate dehydrogenase was mapped at min 7.15 on the Escherichia coli chromosome. This gene (gpsA) is co transduced with the xyl, mtl, and pyrE loci. Three factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosome is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.

Original languageEnglish (US)
Pages (from-to)598-605
Number of pages8
JournalJournal of Bacteriology
Volume118
Issue number2
StatePublished - 1974
Externally publishedYes

Fingerprint

Glycerolphosphate Dehydrogenase
Chromosome Mapping
Phospholipids
Oxidoreductases
Escherichia coli
Membranes
Chromosomes
Genes
Temperature

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

@article{d117a49df8814a9797b64f72efb4f831,
title = "Mutants of Escherichia coli defective in membrane phospholipid synthesis: Mapping of the structural gene for L glycerol 3 phosphate dehydrogenase",
abstract = "The structural gene for the biosynthetic L glycerol 3 phosphate dehydrogenase was mapped at min 7.15 on the Escherichia coli chromosome. This gene (gpsA) is co transduced with the xyl, mtl, and pyrE loci. Three factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosome is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.",
author = "Cronan, {John E} and Bell, {R. M.}",
year = "1974",
language = "English (US)",
volume = "118",
pages = "598--605",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "2",

}

TY - JOUR

T1 - Mutants of Escherichia coli defective in membrane phospholipid synthesis

T2 - Mapping of the structural gene for L glycerol 3 phosphate dehydrogenase

AU - Cronan, John E

AU - Bell, R. M.

PY - 1974

Y1 - 1974

N2 - The structural gene for the biosynthetic L glycerol 3 phosphate dehydrogenase was mapped at min 7.15 on the Escherichia coli chromosome. This gene (gpsA) is co transduced with the xyl, mtl, and pyrE loci. Three factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosome is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.

AB - The structural gene for the biosynthetic L glycerol 3 phosphate dehydrogenase was mapped at min 7.15 on the Escherichia coli chromosome. This gene (gpsA) is co transduced with the xyl, mtl, and pyrE loci. Three factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosome is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.

UR - http://www.scopus.com/inward/record.url?scp=0016237369&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016237369&partnerID=8YFLogxK

M3 - Article

C2 - 4597451

AN - SCOPUS:0016237369

VL - 118

SP - 598

EP - 605

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 2

ER -