TY - JOUR
T1 - Mutants of Escherichia coli defective in membrane phospholipid synthesis
T2 - Mapping of the structural gene for L glycerol 3 phosphate dehydrogenase
AU - Cronan, John E
AU - Bell, R. M.
PY - 1974
Y1 - 1974
N2 - The structural gene for the biosynthetic L glycerol 3 phosphate dehydrogenase was mapped at min 7.15 on the Escherichia coli chromosome. This gene (gpsA) is co transduced with the xyl, mtl, and pyrE loci. Three factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosome is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.
AB - The structural gene for the biosynthetic L glycerol 3 phosphate dehydrogenase was mapped at min 7.15 on the Escherichia coli chromosome. This gene (gpsA) is co transduced with the xyl, mtl, and pyrE loci. Three factor conjugational crosses and the transduction data indicate that the order of loci in this region of the chromosome is mtl, gltE, gpsA, gadR, gadS, pyrE. Study of a temperature sensitive gpsA mutant possessing a dehydrogenase of increased thermolability indicated that gpsA is the structural gene for the dehydrogenase. All dehydrogenase deficient strains tested were mapped very close to the gpsA locus. Attempts at genetic complementation analysis were unsuccessful.
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M3 - Article
C2 - 4597451
AN - SCOPUS:0016237369
VL - 118
SP - 598
EP - 605
JO - Journal of Bacteriology
JF - Journal of Bacteriology
SN - 0021-9193
IS - 2
ER -