Mutagenic responses to ethyl methanesulfonate and phenotypic characterization of an M1 generation of snapdragon, Antirrhinum majus

Leslie M. Heffron, Schuyler S. Korban

Research output: Contribution to journalArticlepeer-review

Abstract

In an effort to induce genetic variations and identify commercially useful variants of snapdragon, seeds of a proprietary snapdragon line OAK564 were treated with different levels (0, 0.10, 0.25, 0.5, 0.75, and 1.0%) of ethyl methanesulfonate (EMS) for three time durations (8, 10, and 12 h). Significant differences were observed for seed germination at 0.25% and 1% EMS, as well as for 0.25% EMS × 8 h and 1% EMS × 12 h treatment combinations. Although there were no significant differences among all EMS treatments and time durations for plant survival, significant differences were observed among EMS treatments and time durations for pollen viability. Based on these findings, a large-scale mutagenesis experiment was conducted using 2000 seeds of line OAK564 treated with either 0.50, 0.75, or 1.0% EMS for 10 h. Germinating seedlings (M1) were evaluated for various phenotypic traits. Several useful alterations in all three M1 populations were observed for plant architecture (dwarfing, multiple branching, and secondary branching), plant color (chlorophyll chimeras, yellowish-green tissues, and dark green tissues), leaf morphology (small leaf size, leaf curling, wavy leaf margins, and thickened striations), and floral morphology (rolled petals, elongated petals, and curtailed petals). Thus, EMS treatments induced various mutations for various phenotypic traits, thereby increasing the genetic diversity in these M1 snapdragon populations.

Original languageEnglish (US)
Article number76
JournalEuphytica
Volume218
Issue number6
DOIs
StatePublished - Jun 2022

Keywords

  • Chemical mutagenesis
  • Genetic variation
  • Mutation breeding
  • Phenotypic characterization
  • Snapdragon

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science
  • Horticulture

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