TY - JOUR
T1 - Mutagenic responses to ethyl methanesulfonate and phenotypic characterization of an M1 generation of snapdragon, Antirrhinum majus
AU - Heffron, Leslie M.
AU - Korban, Schuyler S.
N1 - Funding Information:
This study was supported by the Jonathan Baldwin Turner Graduate Fellowship, a grant received from the Ball Helix (Ball Horticultural, Inc.), the Eugene S. Boerner Scholarship Award, and the ACES Office of Research project 65-325. The authors would like to acknowledge the help and support provided by the late Dr. Alan D. Blowers, Ball Horticultural Co., and would like to thank Dr. Michael Uchneat, then at Ball Horticultural Co. and now at the Huck Institute of Life Sciences at Penn State University for providing seed material and support.
Funding Information:
This study was supported by the University of Illinois at Urbana-Champaign JBT Graduate Fellowship, a grant from Ball Helix (Ball Horticultural, Inc.). and the University of Illinois at Urbana-Champaign Grant No. Project 65-0325.
Funding Information:
This study was supported by the Jonathan Baldwin Turner Graduate Fellowship, a grant received from the Ball Helix (Ball Horticultural, Inc.), the Eugene S. Boerner Scholarship Award, and the ACES Office of Research project 65-325. The authors would like to acknowledge the help and support provided by the late Dr. Alan D. Blowers, Ball Horticultural Co., and would like to thank Dr. Michael Uchneat, then at Ball Horticultural Co. and now at the Huck Institute of Life Sciences at Penn State University for providing seed material and support.
Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer Nature B.V.
PY - 2022/6
Y1 - 2022/6
N2 - In an effort to induce genetic variations and identify commercially useful variants of snapdragon, seeds of a proprietary snapdragon line OAK564 were treated with different levels (0, 0.10, 0.25, 0.5, 0.75, and 1.0%) of ethyl methanesulfonate (EMS) for three time durations (8, 10, and 12 h). Significant differences were observed for seed germination at 0.25% and 1% EMS, as well as for 0.25% EMS × 8 h and 1% EMS × 12 h treatment combinations. Although there were no significant differences among all EMS treatments and time durations for plant survival, significant differences were observed among EMS treatments and time durations for pollen viability. Based on these findings, a large-scale mutagenesis experiment was conducted using 2000 seeds of line OAK564 treated with either 0.50, 0.75, or 1.0% EMS for 10 h. Germinating seedlings (M1) were evaluated for various phenotypic traits. Several useful alterations in all three M1 populations were observed for plant architecture (dwarfing, multiple branching, and secondary branching), plant color (chlorophyll chimeras, yellowish-green tissues, and dark green tissues), leaf morphology (small leaf size, leaf curling, wavy leaf margins, and thickened striations), and floral morphology (rolled petals, elongated petals, and curtailed petals). Thus, EMS treatments induced various mutations for various phenotypic traits, thereby increasing the genetic diversity in these M1 snapdragon populations.
AB - In an effort to induce genetic variations and identify commercially useful variants of snapdragon, seeds of a proprietary snapdragon line OAK564 were treated with different levels (0, 0.10, 0.25, 0.5, 0.75, and 1.0%) of ethyl methanesulfonate (EMS) for three time durations (8, 10, and 12 h). Significant differences were observed for seed germination at 0.25% and 1% EMS, as well as for 0.25% EMS × 8 h and 1% EMS × 12 h treatment combinations. Although there were no significant differences among all EMS treatments and time durations for plant survival, significant differences were observed among EMS treatments and time durations for pollen viability. Based on these findings, a large-scale mutagenesis experiment was conducted using 2000 seeds of line OAK564 treated with either 0.50, 0.75, or 1.0% EMS for 10 h. Germinating seedlings (M1) were evaluated for various phenotypic traits. Several useful alterations in all three M1 populations were observed for plant architecture (dwarfing, multiple branching, and secondary branching), plant color (chlorophyll chimeras, yellowish-green tissues, and dark green tissues), leaf morphology (small leaf size, leaf curling, wavy leaf margins, and thickened striations), and floral morphology (rolled petals, elongated petals, and curtailed petals). Thus, EMS treatments induced various mutations for various phenotypic traits, thereby increasing the genetic diversity in these M1 snapdragon populations.
KW - Chemical mutagenesis
KW - Genetic variation
KW - Mutation breeding
KW - Phenotypic characterization
KW - Snapdragon
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U2 - 10.1007/s10681-022-03034-8
DO - 10.1007/s10681-022-03034-8
M3 - Article
AN - SCOPUS:85130385781
VL - 218
JO - Euphytica
JF - Euphytica
SN - 0014-2336
IS - 6
M1 - 76
ER -