Mutagenic inverted repeats assisted genome engineering (MIRAGE) in saccharomyces cerevisiae: Deletion of gal7

Nikhil U. Nair, Huimin Zhao

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

MIRAGE is a unique in vivo genome editing technique that exploits the inherent instability of inverted repeats (palindromes) in the Saccharomyces cerevisiae chromosome. As a technique able to quickly create deletions as well as precise point mutations, it is valuable in applications that require creation of designer strains of this yeast. In particular, it has various potential applications in metabolic engineering, systems biology, synthetic biology, and molecular genetics.

Original languageEnglish (US)
Title of host publicationMicrobial Metabolic Engineering
Subtitle of host publicationMethods and Protocols
EditorsQiong Cheng
Pages63-73
Number of pages11
DOIs
StatePublished - Jan 20 2012

Publication series

NameMethods in Molecular Biology
Volume834
ISSN (Print)1064-3745

Keywords

  • Homologous recombination
  • In vivo mutagenesis
  • Inverted repeats
  • Metabolic engineering
  • Synthetic biology

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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