Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity

Philip K. Hopke, Michael Jacob Plewa, James B. Johnston, Danny Weaver, Susanne G. Wood, Richard A. Larson, Thomas Hinesly

Research output: Contribution to journalArticle

Abstract

A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.

Original languageEnglish (US)
Pages (from-to)140-147
Number of pages8
JournalEnvironmental Science and Technology
Volume16
Issue number3
DOIs
StatePublished - Jan 1 1982

Fingerprint

Salmonella
Sewage sludge
Acetone
Assays
Screening
Chemical activation
sludge
Cells
germ cell
mutagenicity
cytogenetics
acetone
mutation
assay
damage
screening
sewage sludge
laboratory
in situ
salmonella

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Cite this

Hopke, P. K., Plewa, M. J., Johnston, J. B., Weaver, D., Wood, S. G., Larson, R. A., & Hinesly, T. (1982). Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity. Environmental Science and Technology, 16(3), 140-147. https://doi.org/10.1021/es00097a005

Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity. / Hopke, Philip K.; Plewa, Michael Jacob; Johnston, James B.; Weaver, Danny; Wood, Susanne G.; Larson, Richard A.; Hinesly, Thomas.

In: Environmental Science and Technology, Vol. 16, No. 3, 01.01.1982, p. 140-147.

Research output: Contribution to journalArticle

Hopke, PK, Plewa, MJ, Johnston, JB, Weaver, D, Wood, SG, Larson, RA & Hinesly, T 1982, 'Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity', Environmental Science and Technology, vol. 16, no. 3, pp. 140-147. https://doi.org/10.1021/es00097a005
Hopke, Philip K. ; Plewa, Michael Jacob ; Johnston, James B. ; Weaver, Danny ; Wood, Susanne G. ; Larson, Richard A. ; Hinesly, Thomas. / Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity. In: Environmental Science and Technology. 1982 ; Vol. 16, No. 3. pp. 140-147.
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