Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity

Philip K. Hopke; Michael Jacob Plewa; James B. Johnston; Danny Weaver; Susanne G. Wood; Richard A. Larson; Thomas Hinesly

doi:10.1021/es00097a005

Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity

Philip K. Hopke, Michael Jacob Plewa, James B. Johnston, Danny Weaver, Susanne G. Wood, Richard A. Larson, Thomas Hinesly

Crop Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.

Original language	English (US)
Pages (from-to)	140-147
Number of pages	8
Journal	Environmental Science and Technology
Volume	16
Issue number	3
DOIs	https://doi.org/10.1021/es00097a005
State	Published - Jan 1 1982

ASJC Scopus subject areas

Chemistry(all)
Environmental Chemistry

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title = "Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity",

abstract = "A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.",

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AU - Hopke, Philip K.

AU - Plewa, Michael Jacob

AU - Johnston, James B.

AU - Weaver, Danny

AU - Wood, Susanne G.

AU - Larson, Richard A.

AU - Hinesly, Thomas

PY - 1982/1/1

Y1 - 1982/1/1

N2 - A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.

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