Abstract
A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 140-147 |
| Number of pages | 8 |
| Journal | Environmental Science and Technology |
| Volume | 16 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1982 |
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ASJC Scopus subject areas
- Chemistry(all)
- Environmental Chemistry
Cite this
Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity. / Hopke, Philip K.; Plewa, Michael Jacob; Johnston, James B.; Weaver, Danny; Wood, Susanne G.; Larson, Richard A.; Hinesly, Thomas.
In: Environmental Science and Technology, Vol. 16, No. 3, 01.01.1982, p. 140-147.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multitechnique Screening of Chicago Municipal Sewage Sludge for Mutagenic Activity
AU - Hopke, Philip K.
AU - Plewa, Michael Jacob
AU - Johnston, James B.
AU - Weaver, Danny
AU - Wood, Susanne G.
AU - Larson, Richard A.
AU - Hinesly, Thomas
PY - 1982/1/1
Y1 - 1982/1/1
N2 - A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.
AB - A sample of anaerobically digested Chicago municipal sewage sludge has been examined for mutagenicity by using a variety of biological assays with different genetic end points. The sludge clearly contains components that induce mutagenic responses in all of the species employed. Acetone extracts from the equivalent of 155 µL of neat sludge induced a doubling in the number of revertant colonies of the Ames Salmonella typhimurium strain TA98 after activation with mammalian microsomes. In situ and laboratory studies indicate the induction of point mutations in the germ cells of Zea mays. Cytogenetic damage was demonstrated by the increased frequency of micronuclei observed in meiotic cells of Tradescantia paludosa.
UR - http://www.scopus.com/inward/record.url?scp=0020002584&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0020002584&partnerID=8YFLogxK
U2 - 10.1021/es00097a005
DO - 10.1021/es00097a005
M3 - Article
AN - SCOPUS:0020002584
VL - 16
SP - 140
EP - 147
JO - Environmental Science and Technology
JF - Environmental Science and Technology
SN - 0013-936X
IS - 3
ER -