Multiplexed evaluation of capture agent binding kinetics using arrays of silicon photonic microring resonators

Ji Yeon Byeon, Ryan C. Bailey

Research output: Contribution to journalArticlepeer-review

Abstract

High affinity capture agents recognizing biomolecular targets are essential in the performance of many proteomic detection methods. Herein, we report the application of a label-free silicon photonic biomolecular analysis platform for simultaneously determining kinetic association and dissociation constants for two representative protein capture agents: a thrombin-binding DNA aptamer and an anti-thrombin monoclonal antibody. The scalability and inherent multiplexing capability of the technology make it an attractive platform for simultaneously evaluating the binding characteristics of multiple capture agents recognizing the same target antigen, and thus a tool complementary to emerging high-throughput capture agent generation strategies.

Original languageEnglish (US)
Pages (from-to)3430-3433
Number of pages4
JournalAnalyst
Volume136
Issue number17
DOIs
StatePublished - Sep 7 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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