TY - GEN
T1 - Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone
AU - Sun, Fu
AU - Chen, Weili
AU - Yu, Hojeong
AU - Omob, Akid
AU - Brisbin, Ryan
AU - Ganguli, Anurup
AU - Vemuri, Vinay
AU - Strzebonski, Piotr
AU - Cui, Guangzhe
AU - Allen, Karen J.
AU - Desai, Smit A.
AU - Lin, Weiran
AU - Nash, David M.
AU - Hirschberg, David L.
AU - Brooks, Ian
AU - Bashir, Rashid
AU - Cunningham, Brian T.
N1 - Funding Information:
*Research supported by National Science Foundation (NSF) Fu Sun, Weili Chen, Hojeong Yu, Vinay Vemuri, Piotr Strzebonski, Guangzhe Cui and Weiran Lin are with the Department of Electrical and Computer Engineering; Akid Ornob, Anurup Ganguli, Rashid Bashir and Brian T. Cunningham are with the Department of Bioengineering; Karen J. Allen, Smit A. Desai and Ian Brooks are with the School of Information Sciences, University of Illinois at Urbana-Champaign, IL 61801 USA (corresponding author to provide phone: 217-418-9549; e-mail: fusun2@illinois.edu).
Funding Information:
Research supported by National Science Foundation (NSF)
Funding Information:
ACKNOWLEDGMENT The authors are grateful for the funding support provided by National Science Foundation (NSF) under the grant number 1534126.
Publisher Copyright:
© 2017 IEEE.
PY - 2017/12/19
Y1 - 2017/12/19
N2 - New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.
AB - New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.
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U2 - 10.1109/HIC.2017.8227629
DO - 10.1109/HIC.2017.8227629
M3 - Conference contribution
AN - SCOPUS:85048490093
T3 - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
SP - 241
EP - 244
BT - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Y2 - 6 November 2017 through 8 November 2017
ER -