TY - GEN
T1 - Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone
AU - Sun, Fu
AU - Chen, Weili
AU - Yu, Hojeong
AU - Omob, Akid
AU - Brisbin, Ryan
AU - Ganguli, Anurup
AU - Vemuri, Vinay
AU - Strzebonski, Piotr
AU - Cui, Guangzhe
AU - Allen, Karen J.
AU - Desai, Smit A.
AU - Lin, Weiran
AU - Nash, David M.
AU - Hirschberg, David L.
AU - Brooks, Ian
AU - Bashir, Rashid
AU - Cunningham, Brian T.
N1 - Publisher Copyright:
© 2017 IEEE.
PY - 2017/12/19
Y1 - 2017/12/19
N2 - New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.
AB - New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.
UR - http://www.scopus.com/inward/record.url?scp=85048490093&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85048490093&partnerID=8YFLogxK
U2 - 10.1109/HIC.2017.8227629
DO - 10.1109/HIC.2017.8227629
M3 - Conference contribution
AN - SCOPUS:85048490093
T3 - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
SP - 241
EP - 244
BT - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Y2 - 6 November 2017 through 8 November 2017
ER -