Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone

Fu Sun, Weili Chen, Hojeong Yu, Akid Omob, Ryan Brisbin, Anurup Ganguli, Vinay Vemuri, Piotr Strzebonski, Guangzhe Cui, Karen J. Allen, Smit A. Desai, Weiran Lin, David M. Nash, David L. Hirschberg, Ian Brooks, Rashid Bashir, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

New tools are needed to enable rapid detection, identification, and reporting of infectious viral and microbial pathogens in a wide variety of point-of-care applications that impact human and animal health. We report the design, construction, and characterization of a platform for multiplexed analysis of disease-specific DNA sequences that utilizes a smartphone camera as the sensor in conjunction with a handheld instrument that interfaces the phone with a silicon-based microfluidic chip. Utilizing specific nucleic acid sequences for four equine respiratory pathogens as representative examples, we demonstrated the ability of the system to use a single 15-μL droplet of test sample to perform selective positive/negative determination of target sequences, including integrated experimental controls, in approximately 30 minutes. The system achieves detection limits comparable to those obtained by laboratory-based methods and instruments.

Original languageEnglish (US)
Title of host publication2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages241-244
Number of pages4
ISBN (Electronic)9781538613924
DOIs
StatePublished - Dec 19 2017
Event2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 - Bethesda, United States
Duration: Nov 6 2017Nov 8 2017

Publication series

Name2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
Volume2017-December

Other

Other2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017
CountryUnited States
CityBethesda
Period11/6/1711/8/17

Fingerprint

Point-of-Care Systems
Microfluidics
pathogens
Smartphones
Pathogens
infectious diseases
Silicon
Nucleic Acids
Horses
contagious disease
Communicable Diseases
Amplification
Sequence Analysis
Limit of Detection
Nucleic acid sequences
DNA sequences
Health
nucleic acids
health
animals

ASJC Scopus subject areas

  • Health Informatics
  • Instrumentation
  • Health(social science)
  • Biomedical Engineering

Cite this

Sun, F., Chen, W., Yu, H., Omob, A., Brisbin, R., Ganguli, A., ... Cunningham, B. T. (2017). Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone. In 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017 (pp. 241-244). (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017; Vol. 2017-December). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/HIC.2017.8227629

Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone. / Sun, Fu; Chen, Weili; Yu, Hojeong; Omob, Akid; Brisbin, Ryan; Ganguli, Anurup; Vemuri, Vinay; Strzebonski, Piotr; Cui, Guangzhe; Allen, Karen J.; Desai, Smit A.; Lin, Weiran; Nash, David M.; Hirschberg, David L.; Brooks, Ian; Bashir, Rashid; Cunningham, Brian T.

2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. Institute of Electrical and Electronics Engineers Inc., 2017. p. 241-244 (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017; Vol. 2017-December).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Sun, F, Chen, W, Yu, H, Omob, A, Brisbin, R, Ganguli, A, Vemuri, V, Strzebonski, P, Cui, G, Allen, KJ, Desai, SA, Lin, W, Nash, DM, Hirschberg, DL, Brooks, I, Bashir, R & Cunningham, BT 2017, Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone. in 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017, vol. 2017-December, Institute of Electrical and Electronics Engineers Inc., pp. 241-244, 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017, Bethesda, United States, 11/6/17. https://doi.org/10.1109/HIC.2017.8227629
Sun F, Chen W, Yu H, Omob A, Brisbin R, Ganguli A et al. Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone. In 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. Institute of Electrical and Electronics Engineers Inc. 2017. p. 241-244. (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017). https://doi.org/10.1109/HIC.2017.8227629
Sun, Fu ; Chen, Weili ; Yu, Hojeong ; Omob, Akid ; Brisbin, Ryan ; Ganguli, Anurup ; Vemuri, Vinay ; Strzebonski, Piotr ; Cui, Guangzhe ; Allen, Karen J. ; Desai, Smit A. ; Lin, Weiran ; Nash, David M. ; Hirschberg, David L. ; Brooks, Ian ; Bashir, Rashid ; Cunningham, Brian T. / Multiplexed detection of infectious diseases with microfluidic loop-mediated isothermal amplification and a smartphone. 2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017. Institute of Electrical and Electronics Engineers Inc., 2017. pp. 241-244 (2017 IEEE Healthcare Innovations and Point of Care Technologies, HI-POCT 2017).
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