Multiplex targeting, tracking, and imaging of apoptosis by fluorescent surface enhanced raman spectroscopic dots

Nam Yu Kyeong, Sang Myung Lee, Yun Han Ji, Hyunmi Park, Min Ah Woo, Suk Noh Mi, Soon Kyung Hwang, Jung Taek Kwon, Hua Jin, Yong Kweon Kim, Paul J. Hergenrother, Hong Jeong Dae, Yoon Sik Lee, Myung Haing Cho

Research output: Contribution to journalArticlepeer-review

Abstract

We have developed multifunctional fluorescent surface enhanced Raman spectroscopic tagging material (F-SERS dots) composed of silver nanoparticle-embedded silica spheres with fluorescent organic dye and specific Raman labels for multiplex targeting, tracking, and imaging of cellular/molecular events in the living organism. In this study, F-SERS dots fabricated with specific target antibodies (BAX and BAD) were employed for the detection of apoptosis. The F-SERS dots did not show any particular toxicity in several cell lines. The F-SERS dots could monitor the apoptosis effectively and simultaneously through fluorescent images as well as Raman signals in both cells and tissues with high selectivity. Our results clearly demonstrate that F-SERS dots can be easily applicable to multiplex analysis of diverse cellular/molecular events important for maintaining cellular homeostasis.

Original languageEnglish (US)
Pages (from-to)1155-1162
Number of pages8
JournalBioconjugate Chemistry
Volume18
Issue number4
DOIs
StatePublished - Jul 1 2007

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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