Multiplex serology for common viral infections in feral pigs (Sus scrofa) in Hawaii between 2007 and 2010

Rachel J. Stephenson; Benjamin R. Trible; Y. Wang; Maureen A. Kerrigan; Samuel M. Goldstein; Raymond R.R. Rowland

doi:10.7589/2013-09-242

Multiplex serology for common viral infections in feral pigs (Sus scrofa) in Hawaii between 2007 and 2010

Rachel J. Stephenson, Benjamin R. Trible, Y. Wang, Maureen A. Kerrigan, Samuel M. Goldstein, Raymond R.R. Rowland

Research output: Contribution to journal › Article › peer-review

Abstract

Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations.

Original language	English (US)
Pages (from-to)	239-243
Number of pages	5
Journal	Journal of wildlife diseases
Volume	51
Issue number	1
DOIs	https://doi.org/10.7589/2013-09-242
State	Published - Jan 1 2015
Externally published	Yes

Keywords

Feral pigs
Fluorescence microsphere immunoassay
Hawaii
Porcine circovirus
Porcine reproductive and respiratory syndrome virus
Swine influenza virus

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Ecology

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10.7589/2013-09-242

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title = "Multiplex serology for common viral infections in feral pigs (Sus scrofa) in Hawaii between 2007 and 2010",

abstract = "Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations.",

keywords = "Feral pigs, Fluorescence microsphere immunoassay, Hawaii, Porcine circovirus, Porcine reproductive and respiratory syndrome virus, Swine influenza virus",

author = "Stephenson, {Rachel J.} and Trible, {Benjamin R.} and Y. Wang and Kerrigan, {Maureen A.} and Goldstein, {Samuel M.} and Rowland, {Raymond R.R.}",

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AU - Stephenson, Rachel J.

AU - Trible, Benjamin R.

AU - Wang, Y.

AU - Kerrigan, Maureen A.

AU - Goldstein, Samuel M.

AU - Rowland, Raymond R.R.

N1 - Publisher Copyright: © Wildlife Disease Association 2015.

PY - 2015/1/1

Y1 - 2015/1/1

N2 - Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations.

AB - Multiplex serology was performed for the detection of total immunoglobulin (Ig) and IgM antibodies against porcine circovirus type 2 (PCV2), porcine reproductive and respiratory syndrome virus (PRRSV), and swine influenza virus (SIV) antigens in feral swine (Sus scrofa). Serum samples were collected from the islands of Oahu (292 pigs) and Hawaii (52 pigs) between 2007 and 2010. The highest antibody prevalence was to PCV2 (63%), followed by SIV (7.8%) and PRRSV (5.8%). Antigen-specific IgM was detected at a much lower prevalence. PCR amplification and sequence analysis of PCV2 in three IgM-positive samples identified PCV2b as the only genotype. While the prevalence of PCV2 and PRRSV remained similar between 2007 and 2010, the percentage of SIV-positive samples on Oahu increased from 2% to 19%. Our results demonstrate the utility of multiplex serology for pathogen surveillance in feral pig populations.

KW - Feral pigs

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KW - Porcine circovirus

KW - Porcine reproductive and respiratory syndrome virus

KW - Swine influenza virus

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Multiplex serology for common viral infections in feral pigs (Sus scrofa) in Hawaii between 2007 and 2010

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