TY - JOUR
T1 - Multiple specificities in the repertoire of a melanoma patient's cytolytic T lymphocytes directed against tumor antigen MAGE-1.A1
AU - Romero, Pedro
AU - Pannetier, Christophe
AU - Herman, Jean
AU - Jongeneel, C. Victor
AU - Cerottini, Jean Charles
AU - Coulie, Pierre G.
PY - 1995/10/1
Y1 - 1995/10/1
N2 - Peptide MAGE-1.A1 is a nonamer derived from protein MAGE-1 that can associate with the HLA-A1 molecule. It was shown previously to be recognized an antitumor cytolytic T lymphocyte (CTL) clone derived from the blood of melanoma patient MZ2. We derived two other anti-MAGE-1.A1 CTL clones from different blood samples of the same patient and compared the fine specificity of recognition of the three CTL testing them on variant MAGE-1.A1 peptides incorporating different amino acid substitutions. The epitopes recognized by the CTL proved to be different. While modifications of residues at positions 5, 6, or 7 in the antigenic peptide affected recognition the three CTL, each of the modifications of residues at positions 1, 4, or 8 affected recognition one CTL only. The sequences of both the α and β chains of the T cell antigen receptor of the three CTL were completely different. The results indicate a long-lasting diversity in terms of fine specificity and of T cell antigen receptor structure in the repertoire of antitumor CTL derived from the blood of a melanoma patient and directed against a defined tumor antigen.
AB - Peptide MAGE-1.A1 is a nonamer derived from protein MAGE-1 that can associate with the HLA-A1 molecule. It was shown previously to be recognized an antitumor cytolytic T lymphocyte (CTL) clone derived from the blood of melanoma patient MZ2. We derived two other anti-MAGE-1.A1 CTL clones from different blood samples of the same patient and compared the fine specificity of recognition of the three CTL testing them on variant MAGE-1.A1 peptides incorporating different amino acid substitutions. The epitopes recognized by the CTL proved to be different. While modifications of residues at positions 5, 6, or 7 in the antigenic peptide affected recognition the three CTL, each of the modifications of residues at positions 1, 4, or 8 affected recognition one CTL only. The sequences of both the α and β chains of the T cell antigen receptor of the three CTL were completely different. The results indicate a long-lasting diversity in terms of fine specificity and of T cell antigen receptor structure in the repertoire of antitumor CTL derived from the blood of a melanoma patient and directed against a defined tumor antigen.
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U2 - 10.1084/jem.182.4.1019
DO - 10.1084/jem.182.4.1019
M3 - Article
C2 - 7561675
AN - SCOPUS:0029112509
SN - 0022-1007
VL - 182
SP - 1019
EP - 1028
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
IS - 4
ER -