@article{a104b5a3cadb4a36a8ad8cef2f559e97,
title = "Multiple pathways for glucose phosphate transport and utilization support growth of Cryptosporidium parvum",
abstract = "Cryptosporidium parvum is an obligate intracellular parasite with a highly reduced mitochondrion that lacks the tricarboxylic acid cycle and the ability to generate ATP, making the parasite reliant on glycolysis. Genetic ablation experiments demonstrated that neither of the two putative glucose transporters CpGT1 and CpGT2 were essential for growth. Surprisingly, hexokinase was also dispensable for parasite growth while the downstream enzyme aldolase was required, suggesting the parasite has an alternative way of obtaining phosphorylated hexose. Complementation studies in E. coli support a role for direct transport of glucose-6-phosphate from the host cell by the parasite transporters CpGT1 and CpGT2, thus bypassing a requirement for hexokinase. Additionally, the parasite obtains phosphorylated glucose from amylopectin stores that are released by the action of the essential enzyme glycogen phosphorylase. Collectively, these findings reveal that C. parvum relies on multiple pathways to obtain phosphorylated glucose both for glycolysis and to restore carbohydrate reserves.",
author = "Rui Xu and Beatty, {Wandy L.} and Valentin Greigert and Witola, {William H.} and Sibley, {L. David}",
note = "We thank Daniel Goldberg and members of the Sibley and Goldberg laboratories for helpful advice and Abigail Kimball for assistance with drawing the model. Imaging studies were conducted with assistance from the Molecular Microbiology Imaging Facility. Mass spectrometry was conducted by Sophie Alvarez and Michael Naldrett, Proteomic & Metabolomics Facility (RRID:SCR_021314), Nebraska Center for Biotechnology at the University of Nebraska-Lincoln. The pKD4, pKD46 and pCP20 plasmids were generously gifted by Anna Hooppaw and Mario Feldman in the Department of Molecular Microbiology, at Washington University in St. Louis. The pSC101-AfuABC plasmid was generously gifted by Trevor F. Moraes in the Department of Biochemistry at the University of Toronto. Supported by a grant from the National Institutes of Health (AI145496, LDS). We thank Daniel Goldberg and members of the Sibley and Goldberg laboratories for helpful advice and Abigail Kimball for assistance with drawing the model. Imaging studies were conducted with assistance from the Molecular Microbiology Imaging Facility. Mass spectrometry was conducted by Sophie Alvarez and Michael Naldrett, Proteomic & Metabolomics Facility (RRID:SCR_021314), Nebraska Center for Biotechnology at the University of Nebraska-Lincoln. The pKD4, pKD46 and pCP20 plasmids were generously gifted by Anna Hooppaw and Mario Feldman in the Department of Molecular Microbiology, at Washington University in St. Louis. The pSC101-AfuABC plasmid was generously gifted by Trevor F. Moraes in the Department of Biochemistry at the University of Toronto. Supported by a grant from the National Institutes of Health (AI145496, LDS).",
year = "2024",
month = dec,
doi = "10.1038/s41467-024-44696-3",
language = "English (US)",
volume = "15",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Research",
number = "1",
}