TY - JOUR
T1 - Multiple Genomic Regions Govern Tolerance to Sulfentrazone in Snap Bean (Phaseolus Vulgaris L.)
AU - Saballos, Ana
AU - Soler-Garzón, Alvaro
AU - Brooks, Matthew
AU - Hart, John Patrick
AU - Lipka, Alexander Edward
AU - Miklas, Philip
AU - Peachey, Ronald Edward
AU - Tranel, Patrick J.
AU - Williams, Martin M.
N1 - The authors appreciate Nicholas Hausman for managing the field experiment and numerous students for assisting with data collection; Felix Navarro, Tim Trump, Jen Trapp from Seneca Foods for providing increases of the diversity panel seed; and Jim Meyers from Oregon State University for insightful discussions on this research.
This research was funded by the USDA-Agricultural Research Service, Project number 5012-12220-010-000D, entitled ‘Resilience of Integrated Weed Management Systems to Climate Variability in Midwest Crop Production Systems’, and by an appointment to the Agricultural Research Service Research Participation Program administered by the Oak Ridge Institute for Science and Education through an interagency agreement between the U.S. Department of Energy (DOE) and the USDA.
PY - 2022/6/1
Y1 - 2022/6/1
N2 - The availability of effective weed management tools against waterhemp (Amaranthus tuberculatus) is crucial to maintain profitable production of snap bean (Phaseolus vulgaris L.). Preemergence herbicides enable the crop to gain a size advantage over the weed, but the few preemergence herbicides registered in snap bean have poor control of waterhemp. Sulfentrazone, a protoporphyrinogen oxidase (PPO) -inhibiting herbicide, provides significant control of waterhemp and other problematic weeds. However, crop tolerance to the herbicide is poorly known. To quantify snap bean tolerance to sulfentrazone and investigate the underlying tolerance mechanism(s), a genome-wide association mapping study was conducted using field-collected data on a snap bean diversity panel. Response to a preemergence application of sulfentrazone was measured using plant population density and shoot biomass variables. High levels of crop tolerance were found in several entries including Bush Romano 71, Navarro, and Flamata. Snap bean tolerance to sulfentrazone is associated with multiple genomic regions, indicating the trait is likely a non-target site resistance (NTSR). Seed size is partially responsible for the tolerance, thus the genetic factors conditioning sulfentrazone tolerance are likely a combination of those driven indirectly by seed size/weight and those acting directly on the metabolism of the herbicide or ameliorating its damage. In this study, several cytochrome P450 and ABC transporter genes were localized in associated genomic regions. Alleles conditioning snap bean tolerance to sulfentrazone identified in the diversity panel shed light on herbicide metabolism and could be used in snap bean improvement.
AB - The availability of effective weed management tools against waterhemp (Amaranthus tuberculatus) is crucial to maintain profitable production of snap bean (Phaseolus vulgaris L.). Preemergence herbicides enable the crop to gain a size advantage over the weed, but the few preemergence herbicides registered in snap bean have poor control of waterhemp. Sulfentrazone, a protoporphyrinogen oxidase (PPO) -inhibiting herbicide, provides significant control of waterhemp and other problematic weeds. However, crop tolerance to the herbicide is poorly known. To quantify snap bean tolerance to sulfentrazone and investigate the underlying tolerance mechanism(s), a genome-wide association mapping study was conducted using field-collected data on a snap bean diversity panel. Response to a preemergence application of sulfentrazone was measured using plant population density and shoot biomass variables. High levels of crop tolerance were found in several entries including Bush Romano 71, Navarro, and Flamata. Snap bean tolerance to sulfentrazone is associated with multiple genomic regions, indicating the trait is likely a non-target site resistance (NTSR). Seed size is partially responsible for the tolerance, thus the genetic factors conditioning sulfentrazone tolerance are likely a combination of those driven indirectly by seed size/weight and those acting directly on the metabolism of the herbicide or ameliorating its damage. In this study, several cytochrome P450 and ABC transporter genes were localized in associated genomic regions. Alleles conditioning snap bean tolerance to sulfentrazone identified in the diversity panel shed light on herbicide metabolism and could be used in snap bean improvement.
KW - PPO-inhibiting herbicide
KW - Phaseolus vulgaris
KW - abiotic stress resistance
KW - herbicide tolerance
KW - plant breeding
KW - sulfentrazone
KW - xenobiotic detoxification
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U2 - 10.3389/fagro.2022.869770
DO - 10.3389/fagro.2022.869770
M3 - Article
AN - SCOPUS:85132814871
SN - 2673-3218
VL - 4
JO - Frontiers in Agronomy
JF - Frontiers in Agronomy
M1 - 869770
ER -