TY - GEN
T1 - Multilayered polymer microfluidic chip with nanofluidic interconnects for molecular manipulation
AU - Flachsbart, Bruce R.
AU - Wong, Kachuen
AU - Iannacone, Jamie M.
AU - Abante, Edward N.
AU - Vlach, Robert L.
AU - Rauchfuss, Peter A.
AU - Bohn, Paul W.
AU - Sweedler, Jonathan V.
AU - Shannon, Mark A.
N1 - This work was partiall y supported by National Science Foundation’s hT e Water CAM PWS ,a Science and Technol ogy Cen ter of Advanced Mater ials for the Purification of Water with Systems under the ag eeme nt number C TS-01209 78,the C enter for Nano-Chemical -Electrical-Mechanic al M anufacturing Systems under DM I-032-28162,and by Strategic En vironmental Research and Devel pm ent Prog am under Army W9 132T-05-2-0028.
PY - 2006
Y1 - 2006
N2 - The design, fabrication, and preliminary testing is presented for a polymer multilayered hybrid micro-nanofluidic chip that consists of poly(methylmethacrylate) (PMMA) layers containing microfluidic channels separated in the vertical direction by polycarbonate (PC) nanocapillary array membranes (NCAMs). This design architecture enables nanofluidic interconnections to be placed in the vertical direction between microfluidic channels. Such an architecture combines microfluidic manipulations (separation, injection, collection, etc.) with nanofluidic molecular capabilities (molecular sizing and affinity reactions, channel isolation, enhanced mixing, etc.) on a single chip. Recent polymeric microfabrication advances have made this scalable construct possible: 1) processing thin polymer layers on releasable and compliant carriers, and 2) the high resolution contact-printing of a strong thermal adhesive. Bond strength was demonstrated by pressurizing channels with 90 psi nitrogen without failure. Devices were characterized in terms of measuring resistivity and electroosmotic flow (EOF) along the channels at different pH values. The functionality of the chip is demonstrated by filling a cross channel with 1 µM green-fluorescent protein (GFP) and electrokinetically transporting analyte plugs through the NCAM and down the separation channel while performing laser induced fluorescence (LIF) analysis. The development of this new type of hybrid micro-nanofluidic device potentially will allow unprecedented molecular manipulations for chemical and biological sensing applications.
AB - The design, fabrication, and preliminary testing is presented for a polymer multilayered hybrid micro-nanofluidic chip that consists of poly(methylmethacrylate) (PMMA) layers containing microfluidic channels separated in the vertical direction by polycarbonate (PC) nanocapillary array membranes (NCAMs). This design architecture enables nanofluidic interconnections to be placed in the vertical direction between microfluidic channels. Such an architecture combines microfluidic manipulations (separation, injection, collection, etc.) with nanofluidic molecular capabilities (molecular sizing and affinity reactions, channel isolation, enhanced mixing, etc.) on a single chip. Recent polymeric microfabrication advances have made this scalable construct possible: 1) processing thin polymer layers on releasable and compliant carriers, and 2) the high resolution contact-printing of a strong thermal adhesive. Bond strength was demonstrated by pressurizing channels with 90 psi nitrogen without failure. Devices were characterized in terms of measuring resistivity and electroosmotic flow (EOF) along the channels at different pH values. The functionality of the chip is demonstrated by filling a cross channel with 1 µM green-fluorescent protein (GFP) and electrokinetically transporting analyte plugs through the NCAM and down the separation channel while performing laser induced fluorescence (LIF) analysis. The development of this new type of hybrid micro-nanofluidic device potentially will allow unprecedented molecular manipulations for chemical and biological sensing applications.
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M3 - Conference contribution
AN - SCOPUS:85062360188
T3 - Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop
SP - 197
EP - 200
BT - 2006 Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006
A2 - Kenny, Thomas W.
A2 - Spangler, Leland
PB - Transducer Research Foundation
T2 - 13th Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006
Y2 - 4 June 2006 through 8 June 2006
ER -