Multilayered polymer microfluidic chip with nanofluidic interconnects for molecular manipulation

Bruce R. Flachsbart, Kachuen Wong, Jamie M. Iannacone, Edward N. Abante, Robert L. Vlach, Peter A. Rauchfuss, Paul W. Bohn, Jonathan V. Sweedler, Mark A. Shannon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The design, fabrication, and preliminary testing is presented for a polymer multilayered hybrid micro-nanofluidic chip that consists of poly(methylmethacrylate) (PMMA) layers containing microfluidic channels separated in the vertical direction by polycarbonate (PC) nanocapillary array membranes (NCAMs). This design architecture enables nanofluidic interconnections to be placed in the vertical direction between microfluidic channels. Such an architecture combines microfluidic manipulations (separation, injection, collection, etc.) with nanofluidic molecular capabilities (molecular sizing and affinity reactions, channel isolation, enhanced mixing, etc.) on a single chip. Recent polymeric microfabrication advances have made this scalable construct possible: 1) processing thin polymer layers on releasable and compliant carriers, and 2) the high resolution contact-printing of a strong thermal adhesive. Bond strength was demonstrated by pressurizing channels with 90 psi nitrogen without failure. Devices were characterized in terms of measuring resistivity and electroosmotic flow (EOF) along the channels at different pH values. The functionality of the chip is demonstrated by filling a cross channel with 1 µM green-fluorescent protein (GFP) and electrokinetically transporting analyte plugs through the NCAM and down the separation channel while performing laser induced fluorescence (LIF) analysis. The development of this new type of hybrid micro-nanofluidic device potentially will allow unprecedented molecular manipulations for chemical and biological sensing applications.

Original languageEnglish (US)
Title of host publication2006 Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006
EditorsThomas W. Kenny, Leland Spangler
PublisherTransducer Research Foundation
Pages197-200
Number of pages4
ISBN (Electronic)0964002469, 9780964002463
StatePublished - Jan 1 2006
Event13th Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006 - Hilton Head Island, United States
Duration: Jun 4 2006Jun 8 2006

Publication series

NameTechnical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop

Conference

Conference13th Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006
CountryUnited States
CityHilton Head Island
Period6/4/066/8/06

ASJC Scopus subject areas

  • Control and Systems Engineering
  • Electrical and Electronic Engineering
  • Hardware and Architecture

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  • Cite this

    Flachsbart, B. R., Wong, K., Iannacone, J. M., Abante, E. N., Vlach, R. L., Rauchfuss, P. A., Bohn, P. W., Sweedler, J. V., & Shannon, M. A. (2006). Multilayered polymer microfluidic chip with nanofluidic interconnects for molecular manipulation. In T. W. Kenny, & L. Spangler (Eds.), 2006 Solid-State Sensors, Actuators, and Microsystems Workshop, Hilton Head 2006 (pp. 197-200). (Technical Digest - Solid-State Sensors, Actuators, and Microsystems Workshop). Transducer Research Foundation.