Abstract
Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 3861-3866 |
| Number of pages | 6 |
| Journal | Nano Letters |
| Volume | 12 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 11 2012 |
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Keywords
- SHREC, DNA
- SHRImP
- Super-resolution
- copy-number variations
- genomic variations
- optical DNA mapping
ASJC Scopus subject areas
- Bioengineering
- Chemistry(all)
- Materials Science(all)
- Condensed Matter Physics
- Mechanical Engineering
Cite this
Multicolor super-resolution DNA imaging for genetic analysis. / Baday, Murat; Cravens, Aaron; Hastie, Alex; Kim, Hyeongjun; Kudeki, Deren E.; Kwok, Pui Yan; Xiao, Ming; Selvin, Paul R.
In: Nano Letters, Vol. 12, No. 7, 11.07.2012, p. 3861-3866.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Multicolor super-resolution DNA imaging for genetic analysis
AU - Baday, Murat
AU - Cravens, Aaron
AU - Hastie, Alex
AU - Kim, Hyeongjun
AU - Kudeki, Deren E.
AU - Kwok, Pui Yan
AU - Xiao, Ming
AU - Selvin, Paul R
PY - 2012/7/11
Y1 - 2012/7/11
N2 - Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
AB - Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.
KW - SHREC, DNA
KW - SHRImP
KW - Super-resolution
KW - copy-number variations
KW - genomic variations
KW - optical DNA mapping
UR - http://www.scopus.com/inward/record.url?scp=84863847010&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84863847010&partnerID=8YFLogxK
U2 - 10.1021/nl302069q
DO - 10.1021/nl302069q
M3 - Article
C2 - 22698062
AN - SCOPUS:84863847010
VL - 12
SP - 3861
EP - 3866
JO - Nano Letters
JF - Nano Letters
SN - 1530-6984
IS - 7
ER -