Multicolor super-resolution DNA imaging for genetic analysis

Murat Baday, Aaron Cravens, Alex Hastie, Hyeongjun Kim, Deren E. Kudeki, Pui Yan Kwok, Ming Xiao, Paul R Selvin

Research output: Contribution to journalArticle

Abstract

Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.

Original languageEnglish (US)
Pages (from-to)3861-3866
Number of pages6
JournalNano Letters
Volume12
Issue number7
DOIs
StatePublished - Jul 11 2012

Fingerprint

DNA
deoxyribonucleic acid
Endonucleases
Imaging techniques
enzymes
Enzymes
Genes
Neurodegenerative diseases
genome
abnormalities
imaging techniques
genes
Coloring Agents
Dyes
Diffraction
dyes
cancer
Throughput
costs
high resolution

Keywords

  • SHREC, DNA
  • SHRImP
  • Super-resolution
  • copy-number variations
  • genomic variations
  • optical DNA mapping

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Cite this

Baday, M., Cravens, A., Hastie, A., Kim, H., Kudeki, D. E., Kwok, P. Y., ... Selvin, P. R. (2012). Multicolor super-resolution DNA imaging for genetic analysis. Nano Letters, 12(7), 3861-3866. https://doi.org/10.1021/nl302069q

Multicolor super-resolution DNA imaging for genetic analysis. / Baday, Murat; Cravens, Aaron; Hastie, Alex; Kim, Hyeongjun; Kudeki, Deren E.; Kwok, Pui Yan; Xiao, Ming; Selvin, Paul R.

In: Nano Letters, Vol. 12, No. 7, 11.07.2012, p. 3861-3866.

Research output: Contribution to journalArticle

Baday, M, Cravens, A, Hastie, A, Kim, H, Kudeki, DE, Kwok, PY, Xiao, M & Selvin, PR 2012, 'Multicolor super-resolution DNA imaging for genetic analysis', Nano Letters, vol. 12, no. 7, pp. 3861-3866. https://doi.org/10.1021/nl302069q
Baday M, Cravens A, Hastie A, Kim H, Kudeki DE, Kwok PY et al. Multicolor super-resolution DNA imaging for genetic analysis. Nano Letters. 2012 Jul 11;12(7):3861-3866. https://doi.org/10.1021/nl302069q
Baday, Murat ; Cravens, Aaron ; Hastie, Alex ; Kim, Hyeongjun ; Kudeki, Deren E. ; Kwok, Pui Yan ; Xiao, Ming ; Selvin, Paul R. / Multicolor super-resolution DNA imaging for genetic analysis. In: Nano Letters. 2012 ; Vol. 12, No. 7. pp. 3861-3866.
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