Multicolor super-resolution DNA imaging for genetic analysis

Murat Baday, Aaron Cravens, Alex Hastie, Hyeongjun Kim, Deren E. Kudeki, Pui Yan Kwok, Ming Xiao, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.

Original languageEnglish (US)
Pages (from-to)3861-3866
Number of pages6
JournalNano letters
Volume12
Issue number7
DOIs
StatePublished - Jul 11 2012

Keywords

  • SHREC, DNA
  • SHRImP
  • Super-resolution
  • copy-number variations
  • genomic variations
  • optical DNA mapping

ASJC Scopus subject areas

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

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  • Cite this

    Baday, M., Cravens, A., Hastie, A., Kim, H., Kudeki, D. E., Kwok, P. Y., Xiao, M., & Selvin, P. R. (2012). Multicolor super-resolution DNA imaging for genetic analysis. Nano letters, 12(7), 3861-3866. https://doi.org/10.1021/nl302069q