Multicolor super-resolution DNA imaging for genetic analysis

Murat Baday; Aaron Cravens; Alex Hastie; Hyeongjun Kim; Deren E. Kudeki; Pui Yan Kwok; Ming Xiao; Paul R. Selvin

doi:10.1021/nl302069q

Multicolor super-resolution DNA imaging for genetic analysis

Murat Baday, Aaron Cravens, Alex Hastie, Hyeongjun Kim, Deren E. Kudeki, Pui Yan Kwok, Ming Xiao, Paul R. Selvin

Research output: Contribution to journal › Article

Abstract

Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.

Original language	English (US)
Pages (from-to)	3861-3866
Number of pages	6
Journal	Nano letters
Volume	12
Issue number	7
DOIs	https://doi.org/10.1021/nl302069q
State	Published - Jul 11 2012

Keywords

SHREC, DNA
SHRImP
Super-resolution
copy-number variations
genomic variations
optical DNA mapping

ASJC Scopus subject areas

Bioengineering
Chemistry(all)
Materials Science(all)
Condensed Matter Physics
Mechanical Engineering

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10.1021/nl302069q

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Baday, M., Cravens, A., Hastie, A., Kim, H., Kudeki, D. E., Kwok, P. Y., Xiao, M., & Selvin, P. R. (2012). Multicolor super-resolution DNA imaging for genetic analysis. Nano letters, 12(7), 3861-3866. https://doi.org/10.1021/nl302069q

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abstract = "Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.",

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AU - Baday, Murat

AU - Cravens, Aaron

AU - Hastie, Alex

AU - Kim, Hyeongjun

AU - Kudeki, Deren E.

AU - Kwok, Pui Yan

AU - Xiao, Ming

AU - Selvin, Paul R.

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AB - Many types of cancer and neurodegenerative diseases are caused by abnormalities and variations in the genome. We have designed a high-resolution imaging technique with high throughput and low cost for determining structural variations of genes related to genetic diseases. We initially mapped all seven nicking sites of Nb.BbvCI endonuclease enzyme on lambda DNA. Then we resolved densely labeled patterns of 107 nicking sites on human BAC DNA that is digested by Nb.BsmI and Nb.BbvCI endonuclease enzymes. This high density resulted in several dyes being closer together than the diffraction limit. Overall, detailed DNA nicking sites mapping with 100bp resolution was achieved, which has the potential to reveal information about genetic variance and to facilitate medical diagnosis of several genetic diseases.

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