Multicolor Discrete Frequency Infrared Spectroscopic Imaging

Kevin Yeh, Dongkwan Lee, Rohit Bhargava

Research output: Contribution to journalArticle

Abstract

Advancement of discrete frequency infrared (DFIR) spectroscopic microscopes in image quality and data throughput are critical to their use for analytical measurements. Here, we report the development and characterization of a point scanning instrument with minimal aberrations and capable of diffraction-limited performance across all fingerprint region wavelengths over arbitrarily large samples. The performance of this system is compared to commercial state of the art Fourier transform infrared (FT-IR) imaging systems. We show that for large samples or smaller set of discrete frequencies, point scanning far exceeds (10-100 fold) comparable data acquired with FT-IR instruments. Further we show improvements in image quality using refractive lenses that show significantly improved contrast across the spatial frequency bandwidth. Finally, we introduce the ability to image two tunable frequencies simultaneously using a single detector by means of demodulation to further speed up data acquisition and reduce the impact of scattering. Together, the advancements provide significantly better spectral quality and spatial fidelity than current state of the art imaging systems while promising to make spectral scanning even faster.

Original languageEnglish (US)
Pages (from-to)2177-2185
Number of pages9
JournalAnalytical chemistry
Volume91
Issue number3
DOIs
StatePublished - Feb 5 2019

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Infrared radiation
Scanning
Imaging techniques
Imaging systems
Image quality
Fourier transforms
Infrared instruments
Infrared imaging
Demodulation
Aberrations
Lenses
Data acquisition
Microscopes
Diffraction
Throughput
Scattering
Detectors
Bandwidth
Wavelength

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Multicolor Discrete Frequency Infrared Spectroscopic Imaging. / Yeh, Kevin; Lee, Dongkwan; Bhargava, Rohit.

In: Analytical chemistry, Vol. 91, No. 3, 05.02.2019, p. 2177-2185.

Research output: Contribution to journalArticle

Yeh, Kevin ; Lee, Dongkwan ; Bhargava, Rohit. / Multicolor Discrete Frequency Infrared Spectroscopic Imaging. In: Analytical chemistry. 2019 ; Vol. 91, No. 3. pp. 2177-2185.
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