TY - JOUR
T1 - Multiassay nutritional metabolomics profiling of low vitamin A status versus adequacy is characterized by reduced plasma lipid mediators among lactating women in the Philippines
T2 - A pilot study
AU - Johnson, Catherine M.
AU - Rosario, Rodrigo
AU - Brito, Alex
AU - Agrawal, Karan
AU - Fanter, Rob
AU - Lietz, Georg
AU - Haskell, Marjorie
AU - Engle-Stone, Reina
AU - Newman, John W.
AU - La Frano, Michael R.
N1 - Funding from NIH U24 DK097154, NIH T32-GM008799, and the Cal Poly College of Agriculture Food and Environmental Sciences (CAFES) Summer Undergraduate Research Program (SURP) primarily supported this research. A.B. was supported by the Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers “Digital Biodesign and Personalized Healthcare No. 075-15-2020-926.” Additional support was provided to J.W.N. by the USDA (Intramural Projects 2032-51530-022-00D and 2032-51530-025-00D). The contents of this manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the NIGMS, NIH, or USDA. The USDA is an equal opportunity employer and provider.
We thank Akoto Osei, Maria Fatima Dolly Reario, and Eleanore LaFuente (Helen Keller International) and Dr. Nestor Cailo for their contributions to data collection.
PY - 2022/8
Y1 - 2022/8
N2 - Low vitamin A (VA) status is common among lactating women in low-income countries. Lactation has substantial effects on mother's metabolism and VA is required in multiple biological processes, including growth, vision, immunity, and reproduction. The objective of this pilot study was to use metabolomics profiling to conduct a broad, exploratory assessment of differences in plasma metabolites associated with low VA status versus VA adequacy in lactating women. Plasma samples from lactating women who participated in a survey in Samar, Philippines, were selected from a cross-sectional study based on plasma retinol concentrations indicating low (VA–; n = 5) or adequate (VA+; n = 5) VA status (plasma retinol <0.8 or >1.05 µmol/L). The plasma results collected from 6 metabolomics assays (oxylipins, endocannabinoids, bile acids, primary metabolomics, biogenic amines, and lipidomics) were compared by group using liquid chromatography mass spectrometry. Twenty-eight metabolites were altered in the VA– versus VA+ status groups, with 24 being lipid mediators (P < .05). These lipid mediators included lower concentrations of arachidonic acid- and eicosapentaenoic acid-derived oxylipins, as well as lysophospholipids and sphingolipids, in the VA– group (P < .05). Chemical similarity enrichment analysis identified hydroxy-eicosatetraenoic acids, hydroxy-eicosapentaenoic acids, and dihydroxy-eicosatetraenoic acids as significantly altered oxylipin clusters (P < .0001, false discovery rate [FDR] P < .0001), as well as sphingomyelins, saturated lysophosphatidylcholines, phosphatidylcholines, and phosphatidylethanolamines (P < .001, FDR P < .01). The multiassay nutritional metabolomics profiling of low VA status compared with adequacy in lactating women was characterized by reduced lipid mediator concentrations. Future studies with stronger study designs and larger sample size are needed to confirm and validate these preliminary results.
AB - Low vitamin A (VA) status is common among lactating women in low-income countries. Lactation has substantial effects on mother's metabolism and VA is required in multiple biological processes, including growth, vision, immunity, and reproduction. The objective of this pilot study was to use metabolomics profiling to conduct a broad, exploratory assessment of differences in plasma metabolites associated with low VA status versus VA adequacy in lactating women. Plasma samples from lactating women who participated in a survey in Samar, Philippines, were selected from a cross-sectional study based on plasma retinol concentrations indicating low (VA–; n = 5) or adequate (VA+; n = 5) VA status (plasma retinol <0.8 or >1.05 µmol/L). The plasma results collected from 6 metabolomics assays (oxylipins, endocannabinoids, bile acids, primary metabolomics, biogenic amines, and lipidomics) were compared by group using liquid chromatography mass spectrometry. Twenty-eight metabolites were altered in the VA– versus VA+ status groups, with 24 being lipid mediators (P < .05). These lipid mediators included lower concentrations of arachidonic acid- and eicosapentaenoic acid-derived oxylipins, as well as lysophospholipids and sphingolipids, in the VA– group (P < .05). Chemical similarity enrichment analysis identified hydroxy-eicosatetraenoic acids, hydroxy-eicosapentaenoic acids, and dihydroxy-eicosatetraenoic acids as significantly altered oxylipin clusters (P < .0001, false discovery rate [FDR] P < .0001), as well as sphingomyelins, saturated lysophosphatidylcholines, phosphatidylcholines, and phosphatidylethanolamines (P < .001, FDR P < .01). The multiassay nutritional metabolomics profiling of low VA status compared with adequacy in lactating women was characterized by reduced lipid mediator concentrations. Future studies with stronger study designs and larger sample size are needed to confirm and validate these preliminary results.
KW - Lipid mediators
KW - Metabolomics
KW - Micronutrient deficiencies
KW - Nutritional metabolomics
KW - Oxylipins
KW - Vitamin A
UR - http://www.scopus.com/inward/record.url?scp=85132739498&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85132739498&partnerID=8YFLogxK
U2 - 10.1016/j.nutres.2022.05.007
DO - 10.1016/j.nutres.2022.05.007
M3 - Article
C2 - 35732076
AN - SCOPUS:85132739498
SN - 0271-5317
VL - 104
SP - 118
EP - 127
JO - Nutrition Research
JF - Nutrition Research
ER -