Multi-modality Imaging of structure and function combining spectral-domain optical coherence and multiphoton microscopy

Claudio Vinegoni, Tyler Ralston, Wei Tan, Wei Luo, Daniel L. Marks, Stephen A. Boppart

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We have constructed an integrated optical microscope that allows for the simultaneous image acquisition from multiple optical imaging modalities. The microscope consists primarily of hardware for spectral-domain optical coherence microscopy (OCM), multi-photon microscopy (MPM), and second harmonic generation microscopy. The unique configuration of the integrated microscope allows for the acquisition of both anatomical and functional imaging information with particular emphasis in the fields of tissue engineering and tumor biology. By overlaying the structural image obtained from OCM on the functional image obtained simultaneously from MPM (i.e., fluorescent markers imply functional proteins), a more comprehensive view of different tissues can be obtained. In addition, the contemporary analysis of the spectroscopic features enhances contrast in OCM by differentiating different cell and tissue components.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
DOIs
StatePublished - 2006
EventCoherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X - San Jose, CA, United States
Duration: Jan 23 2006Jan 25 2006

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6079
ISSN (Print)0277-786X

Other

OtherCoherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine X
Country/TerritoryUnited States
CitySan Jose, CA
Period1/23/061/25/06

Keywords

  • Multiphoton Microscopy
  • Optical Coherence Microscopy
  • Spectral-Domain Detection
  • Spectroscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Fingerprint

Dive into the research topics of 'Multi-modality Imaging of structure and function combining spectral-domain optical coherence and multiphoton microscopy'. Together they form a unique fingerprint.

Cite this