Abstract

Cell-based biohybrid actuators are integrated systems that use biological components including proteins and cells to power material components by converting chemical energy to mechanical energy. The latest progress in cell-based biohybrid actuators has been limited to rigid materials, such as silicon and PDMS, ranging in elastic moduli on the order of mega (10 6) to giga (10 9) Pascals. Recent reports in the literature have established a correlation between substrate rigidity and its influence on the contractile behavior of cardiomyocytes (A. J. Engler, C. Carag-Krieger, C. P. Johnson, M. Raab, H. Y. Tang and D. W. Speicher, et al., J. Cell Sci., 2008, 121(Pt 22), 3794-3802, P. Bajaj, X. Tang, T. A. Saif and R. Bashir, J. Biomed. Mater. Res., Part A, 2010, 95(4), 1261-1269). This study explores the fabrication of a more compliant cantilever, similar to that of the native myocardium, with elasticity on the order of kilo (10 3) Pascals. 3D stereolithographic technology, a layer-by-layer UV polymerizable rapid prototyping system, was used to rapidly fabricate multi-material cantilevers composed of poly(ethylene glycol) diacrylate (PEGDA) and acrylic-PEG-collagen (PC) mixtures. The incorporation of acrylic-PEG-collagen into PEGDA-based materials enhanced cell adhesion, spreading, and organization without altering the ability to vary the elastic modulus through the molecular weight of PEGDA. Cardiomyocytes derived from neonatal rats were seeded on the cantilevers, and the resulting stresses and contractile forces were calculated using finite element simulations validated with classical beam equations. These cantilevers can be used as a mechanical sensor to measure the contractile forces of cardiomyocyte cell sheets, and as an early prototype for the design of optimal cell-based biohybrid actuators.

Original languageEnglish (US)
Pages (from-to)88-98
Number of pages11
JournalLab on a Chip
Volume12
Issue number1
DOIs
StatePublished - Jan 7 2012

Fingerprint

Stereolithography
Hydrogel
Hydrogels
Polyethylene glycols
Actuators
Fabrication
Cardiac Myocytes
Acrylics
Collagen
Elastic Modulus
Elastic moduli
Cell adhesion
Rapid prototyping
Silicon
Rigidity
Rats
Elasticity
Aptitude
Molecular weight
Cell Adhesion

ASJC Scopus subject areas

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

Cite this

Multi-material bio-fabrication of hydrogel cantilevers and actuators with stereolithography. / Chan, Vincent; Jeong, Jae Hyun; Bajaj, Piyush; Collens, Mitchell; Saif, Taher; Kong, Hyunjoon; Bashir, Rashid.

In: Lab on a Chip, Vol. 12, No. 1, 07.01.2012, p. 88-98.

Research output: Contribution to journalArticle

Chan, Vincent ; Jeong, Jae Hyun ; Bajaj, Piyush ; Collens, Mitchell ; Saif, Taher ; Kong, Hyunjoon ; Bashir, Rashid. / Multi-material bio-fabrication of hydrogel cantilevers and actuators with stereolithography. In: Lab on a Chip. 2012 ; Vol. 12, No. 1. pp. 88-98.
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