Multi-color fluorescence enhancement from a photonics crystal surface

A. Pokhriyal, M. Lu, S. Schulz, C. S. Huang, B. T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ∼3×5 in2 surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ = 632.8 nm laser (cyanine-5) and a dye excited by a λ = 532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32x increase in fluorescent signal intensity for cyanine-5 conjugated strepavidin labeling, while a 25x increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm < λ < 633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.

Original languageEnglish (US)
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages2287-2290
Number of pages4
DOIs
StatePublished - Dec 1 2010
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: Nov 1 2010Nov 4 2010

Publication series

NameProceedings of IEEE Sensors

Other

Other9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period11/1/1011/4/10

Fingerprint

Photonic crystals
Dyes
Fluorescence
Color
Labeling
Wavelength
Laser excitation
Fluorophores
Lasers
Microarrays
Gene expression
Molding
Assays
Electric fields
Plastics
Proteins
Glass
Substrates

ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Pokhriyal, A., Lu, M., Schulz, S., Huang, C. S., & Cunningham, B. T. (2010). Multi-color fluorescence enhancement from a photonics crystal surface. In IEEE Sensors 2010 Conference, SENSORS 2010 (pp. 2287-2290). [5690806] (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2010.5690806

Multi-color fluorescence enhancement from a photonics crystal surface. / Pokhriyal, A.; Lu, M.; Schulz, S.; Huang, C. S.; Cunningham, B. T.

IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 2287-2290 5690806 (Proceedings of IEEE Sensors).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pokhriyal, A, Lu, M, Schulz, S, Huang, CS & Cunningham, BT 2010, Multi-color fluorescence enhancement from a photonics crystal surface. in IEEE Sensors 2010 Conference, SENSORS 2010., 5690806, Proceedings of IEEE Sensors, pp. 2287-2290, 9th IEEE Sensors Conference 2010, SENSORS 2010, Waikoloa, HI, United States, 11/1/10. https://doi.org/10.1109/ICSENS.2010.5690806
Pokhriyal A, Lu M, Schulz S, Huang CS, Cunningham BT. Multi-color fluorescence enhancement from a photonics crystal surface. In IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 2287-2290. 5690806. (Proceedings of IEEE Sensors). https://doi.org/10.1109/ICSENS.2010.5690806
Pokhriyal, A. ; Lu, M. ; Schulz, S. ; Huang, C. S. ; Cunningham, B. T. / Multi-color fluorescence enhancement from a photonics crystal surface. IEEE Sensors 2010 Conference, SENSORS 2010. 2010. pp. 2287-2290 (Proceedings of IEEE Sensors).
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