Abstract
A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ∼3×5 in2 surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ = 632.8 nm laser (cyanine-5) and a dye excited by a λ = 532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32x increase in fluorescent signal intensity for cyanine-5 conjugated strepavidin labeling, while a 25x increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm < λ < 633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.
| Original language | English (US) |
|---|---|
| Title of host publication | IEEE Sensors 2010 Conference, SENSORS 2010 |
| Pages | 2287-2290 |
| Number of pages | 4 |
| DOIs | |
| State | Published - Dec 1 2010 |
| Event | 9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States Duration: Nov 1 2010 → Nov 4 2010 |
Publication series
| Name | Proceedings of IEEE Sensors |
|---|
Other
| Other | 9th IEEE Sensors Conference 2010, SENSORS 2010 |
|---|---|
| Country | United States |
| City | Waikoloa, HI |
| Period | 11/1/10 → 11/4/10 |
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ASJC Scopus subject areas
- Electrical and Electronic Engineering
Cite this
Multi-color fluorescence enhancement from a photonics crystal surface. / Pokhriyal, A.; Lu, M.; Schulz, S.; Huang, C. S.; Cunningham, B. T.
IEEE Sensors 2010 Conference, SENSORS 2010. 2010. p. 2287-2290 5690806 (Proceedings of IEEE Sensors).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution
}
TY - GEN
T1 - Multi-color fluorescence enhancement from a photonics crystal surface
AU - Pokhriyal, A.
AU - Lu, M.
AU - Schulz, S.
AU - Huang, C. S.
AU - Cunningham, B. T.
PY - 2010/12/1
Y1 - 2010/12/1
N2 - A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ∼3×5 in2 surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ = 632.8 nm laser (cyanine-5) and a dye excited by a λ = 532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32x increase in fluorescent signal intensity for cyanine-5 conjugated strepavidin labeling, while a 25x increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm < λ < 633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.
AB - A photonic crystal substrate exhibiting resonant enhancement of multiple fluorophores has been demonstrated. The device, fabricated uniformly from plastic materials over a ∼3×5 in2 surface area by nanoreplica molding, utilizes two distinct resonant modes to enhance electric field stimulation of a dye excited by a λ = 632.8 nm laser (cyanine-5) and a dye excited by a λ = 532 nm laser (cyanine-3). Resonant coupling of the laser excitation to the photonic crystal surface is obtained for each wavelength at a distinct incident angle. Compared to detection of a dye-labeled protein on an ordinary glass surface, the photonic crystal surface exhibited a 32x increase in fluorescent signal intensity for cyanine-5 conjugated strepavidin labeling, while a 25x increase was obtained for cyanine-3 conjugated streptavidin labeling. The photonic crystal is capable of amplifying the output of any fluorescent dye with an excitation wavelength in the 532 nm < λ < 633 nm range by selection of an appropriate incident angle. The device is designed for biological assays that utilize multiple fluorescent dyes within a single imaged area, such as gene expression microarrays.
UR - http://www.scopus.com/inward/record.url?scp=79951902251&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=79951902251&partnerID=8YFLogxK
U2 - 10.1109/ICSENS.2010.5690806
DO - 10.1109/ICSENS.2010.5690806
M3 - Conference contribution
AN - SCOPUS:79951902251
SN - 9781424481682
T3 - Proceedings of IEEE Sensors
SP - 2287
EP - 2290
BT - IEEE Sensors 2010 Conference, SENSORS 2010
ER -