Mouse nestin protein localizes in growth cones of P19 neurons and cerebellar granule cells

Ye Yan, Jing Yang, Wei Bian, Naihe Jing

Research output: Contribution to journalArticlepeer-review

Abstract

The neuronal growth cone, a highly motile structure at the distal tip of growing axons, contains filamentous actin and microtubules as its main cytoskeletal components. Using immunocytochemistry, we observed that nestin, which is the predominant intermediate filament protein in neuroepithelial cells and young neurons of the developing brain, appears to be strongly expressed in neurites and growth cones of neurons differentiating from P19 embryonic carcinoma cells in vitro. Double-staining of nestin and microtubule-associated protein-2 as well as nestin and growth-associated protein-43 revealed that nestin protein localizes in neurites and the central regions of growth cones of primary cultures of cerebellar granule cells from postnatal day 6 mice. These results suggest a role for nestin in growth cone guidance during axon elongation.

Original languageEnglish (US)
Pages (from-to)89-92
Number of pages4
JournalNeuroscience Letters
Volume302
Issue number2-3
DOIs
StatePublished - Apr 20 2001
Externally publishedYes

Keywords

  • Cerebellar granule cells
  • Growth cone
  • Growth-associated protein-43
  • Nestin
  • P19 neurons

ASJC Scopus subject areas

  • Neuroscience(all)

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