Mouse nestin cDNA cloning and protein expression in the cytoskeleton of transfected cells

Jing Yang; Leping Cheng; Ye Yan; Wei Bian; Yasuhiro Tomooka; Robert Shiurba; Naihe Jing

doi:10.1016/S0167-4781(01)00275-5

Mouse nestin cDNA cloning and protein expression in the cytoskeleton of transfected cells

Jing Yang, Leping Cheng, Ye Yan, Wei Bian, Yasuhiro Tomooka, Robert Shiurba, Naihe Jing

Research output: Contribution to journal › Article › peer-review

Abstract

Complementary DNA (cDNA) corresponding to mouse nestin intermediate filament protein, a specific marker for neural stem cells, was isolated and characterized. The complete sequence comprised 5983 base pairs encoding 1821 amino acids, and the deduced polypeptide was similar to rat (84%), hamster (73%), and human (62%) nestin. Southern blots showed that mouse nestin was a single-copy gene, and Northern blots detected a 6.0 kilobase mRNA transcript. When the cDNA was overexpressed as an enhanced green fluorescent fusion protein in COS7 cells, nestin immunoreactivity appeared in the filamentous cytoskeletal network. Accordingly, biologically active mouse nestin cDNA may offer an important new tool for stem cell research.

Original language	English (US)
Pages (from-to)	251-254
Number of pages	4
Journal	Biochimica et Biophysica Acta - Gene Structure and Expression
Volume	1520
Issue number	3
DOIs	https://doi.org/10.1016/S0167-4781(01)00275-5
State	Published - Sep 21 2001
Externally published	Yes

Keywords

Cytoskeleton
Mouse
Nestin
cDNA cloning

ASJC Scopus subject areas

Structural Biology
Biophysics
Biochemistry
Genetics

Online availability

10.1016/S0167-4781(01)00275-5

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title = "Mouse nestin cDNA cloning and protein expression in the cytoskeleton of transfected cells",

abstract = "Complementary DNA (cDNA) corresponding to mouse nestin intermediate filament protein, a specific marker for neural stem cells, was isolated and characterized. The complete sequence comprised 5983 base pairs encoding 1821 amino acids, and the deduced polypeptide was similar to rat (84%), hamster (73%), and human (62%) nestin. Southern blots showed that mouse nestin was a single-copy gene, and Northern blots detected a 6.0 kilobase mRNA transcript. When the cDNA was overexpressed as an enhanced green fluorescent fusion protein in COS7 cells, nestin immunoreactivity appeared in the filamentous cytoskeletal network. Accordingly, biologically active mouse nestin cDNA may offer an important new tool for stem cell research.",

keywords = "Cytoskeleton, Mouse, Nestin, cDNA cloning",

author = "Jing Yang and Leping Cheng and Ye Yan and Wei Bian and Yasuhiro Tomooka and Robert Shiurba and Naihe Jing",

note = "Funding Information: We thank P. Chambon for the E10 mouse cDNA library and S. Kumar for the mouse neural precursor cell cDNA library. This work was supported by grants from the National Basic Research Program (G 1999054000) and National Natural Science Foundation of China (39870283, 39930090).",

year = "2001",

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doi = "10.1016/S0167-4781(01)00275-5",

language = "English (US)",

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pages = "251--254",

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TY - JOUR

T1 - Mouse nestin cDNA cloning and protein expression in the cytoskeleton of transfected cells

AU - Yang, Jing

AU - Cheng, Leping

AU - Yan, Ye

AU - Bian, Wei

AU - Tomooka, Yasuhiro

AU - Shiurba, Robert

AU - Jing, Naihe

N1 - Funding Information: We thank P. Chambon for the E10 mouse cDNA library and S. Kumar for the mouse neural precursor cell cDNA library. This work was supported by grants from the National Basic Research Program (G 1999054000) and National Natural Science Foundation of China (39870283, 39930090).

PY - 2001/9/21

Y1 - 2001/9/21

N2 - Complementary DNA (cDNA) corresponding to mouse nestin intermediate filament protein, a specific marker for neural stem cells, was isolated and characterized. The complete sequence comprised 5983 base pairs encoding 1821 amino acids, and the deduced polypeptide was similar to rat (84%), hamster (73%), and human (62%) nestin. Southern blots showed that mouse nestin was a single-copy gene, and Northern blots detected a 6.0 kilobase mRNA transcript. When the cDNA was overexpressed as an enhanced green fluorescent fusion protein in COS7 cells, nestin immunoreactivity appeared in the filamentous cytoskeletal network. Accordingly, biologically active mouse nestin cDNA may offer an important new tool for stem cell research.

AB - Complementary DNA (cDNA) corresponding to mouse nestin intermediate filament protein, a specific marker for neural stem cells, was isolated and characterized. The complete sequence comprised 5983 base pairs encoding 1821 amino acids, and the deduced polypeptide was similar to rat (84%), hamster (73%), and human (62%) nestin. Southern blots showed that mouse nestin was a single-copy gene, and Northern blots detected a 6.0 kilobase mRNA transcript. When the cDNA was overexpressed as an enhanced green fluorescent fusion protein in COS7 cells, nestin immunoreactivity appeared in the filamentous cytoskeletal network. Accordingly, biologically active mouse nestin cDNA may offer an important new tool for stem cell research.

KW - Cytoskeleton

KW - Mouse

KW - Nestin

KW - cDNA cloning

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AN - SCOPUS:0035929414

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SP - 251

EP - 254

JO - Biochimica et Biophysica Acta - Gene Structure and Expression

JF - Biochimica et Biophysica Acta - Gene Structure and Expression

IS - 3

ER -

Mouse nestin cDNA cloning and protein expression in the cytoskeleton of transfected cells

Abstract

Keywords

ASJC Scopus subject areas

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