Most influenza A virions fail to express at least one essential viral protein

Christopher B. Brooke, William L. Ince, Jens Wrammert, Rafi Ahmed, Patrick C. Wilson, Jack R. Bennink, Jonathan W. Yewdell

Research output: Contribution to journalArticle

Abstract

Segmentation of the influenza A virus (IAV) genome enables rapid gene reassortment at the cost of complicating the task of assembling the full viral genome. By simultaneously probing for the expression of multiple viral proteins in MDCK cells infected at a low multiplicity with IAV, we observe that the majority of infected cells lack detectable expression of one or more essential viral proteins. Consistent with this observation, up to 90% of IAV-infected cells fail to release infectious progeny, indicating that many IAV virions scored as noninfectious by traditional infectivity assays are capable of single-round infection. This fraction was not significantly affected by target or producer cell type but varied widely between different IAV strains. These data indicate that IAV exists primarily as a swarm of complementation-dependent semi-infectious virions, and thus traditional, propagationdependent assays of infectivity may drastically misrepresent the true infectious potential of a virus population.

Original languageEnglish (US)
Pages (from-to)3155-3162
Number of pages8
JournalJournal of virology
Volume87
Issue number6
DOIs
StatePublished - Mar 1 2013
Externally publishedYes

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viral proteins
Influenza A virus
Viral Proteins
virion
influenza
Virion
Human Influenza
pathogenicity
cells
Madin Darby Canine Kidney Cells
genome
Viral Genome
swarms
assays
Genome
Viruses
viruses
Infection
infection
Population

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Brooke, C. B., Ince, W. L., Wrammert, J., Ahmed, R., Wilson, P. C., Bennink, J. R., & Yewdell, J. W. (2013). Most influenza A virions fail to express at least one essential viral protein. Journal of virology, 87(6), 3155-3162. https://doi.org/10.1128/JVI.02284-12

Most influenza A virions fail to express at least one essential viral protein. / Brooke, Christopher B.; Ince, William L.; Wrammert, Jens; Ahmed, Rafi; Wilson, Patrick C.; Bennink, Jack R.; Yewdell, Jonathan W.

In: Journal of virology, Vol. 87, No. 6, 01.03.2013, p. 3155-3162.

Research output: Contribution to journalArticle

Brooke, CB, Ince, WL, Wrammert, J, Ahmed, R, Wilson, PC, Bennink, JR & Yewdell, JW 2013, 'Most influenza A virions fail to express at least one essential viral protein', Journal of virology, vol. 87, no. 6, pp. 3155-3162. https://doi.org/10.1128/JVI.02284-12
Brooke, Christopher B. ; Ince, William L. ; Wrammert, Jens ; Ahmed, Rafi ; Wilson, Patrick C. ; Bennink, Jack R. ; Yewdell, Jonathan W. / Most influenza A virions fail to express at least one essential viral protein. In: Journal of virology. 2013 ; Vol. 87, No. 6. pp. 3155-3162.
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