Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae)

Yi Chun Tsai, Leellen F. Solter, Chih Yuan Wang, Huai Sheng Fan, Chun che Chang, Chung Hsiung Wang

Research output: Contribution to journalArticle

Abstract

A microsporidium possessing molecular and morphological characteristics of the genus Nosema was isolated from larvae of the thee-spot grass yellow butterfly, Eurema blanda arsakia. The complete rRNA gene sequences of the E. blanda isolate contained 4,428 base pairs (GenBank Accession No. EU338534). The organization of the rRNA genes is LSU rRNA-ITS-SSU rRNA-IGS-5S, which corresponds with that of Nosema species closely related to Nosema bombycis. Phylogenetic analysis based on rRNA gene sequences show that this isolate is closely related to Nosema bombycis, Nosema plutellae, Nosema spodopterae, and Nosema antheraeae. The ultrastructure of all developmental stages of this microsporidium confirmed its placement in the genus Nosema. The isolate was successfully propagated in cell lines IPLB-LD652Y (Lymantria dispar) and NTU-LY (Lymantria xylina) and, in the in vitro system, it was frequently found to develop in the nuclei of the host cells, a circumstance that seldom occurs in other Nosema species. An extra-cellular vegetative stage of this microsporidium was also observed in the culture medium after 14 days of infection. The ECMDFs might be released from disrupted host cells.

Original languageEnglish (US)
Pages (from-to)85-93
Number of pages9
JournalJournal of Invertebrate Pathology
Volume100
Issue number2
DOIs
StatePublished - Feb 1 2009

Keywords

  • Extra-cellular microsporidian developmental forms
  • Intra-nuclear development
  • Microsporidium
  • Nosema
  • Phylogenetic analysis
  • RRNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics

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