Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae)

Yi Chun Tsai; Leellen F. Solter; Chih Yuan Wang; Huai Sheng Fan; Chun che Chang; Chung Hsiung Wang

doi:10.1016/j.jip.2008.11.006

Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae)

Yi Chun Tsai, Leellen F. Solter, Chih Yuan Wang, Huai Sheng Fan, Chun che Chang, Chung Hsiung Wang

Research output: Contribution to journal › Article › peer-review

Abstract

A microsporidium possessing molecular and morphological characteristics of the genus Nosema was isolated from larvae of the thee-spot grass yellow butterfly, Eurema blanda arsakia. The complete rRNA gene sequences of the E. blanda isolate contained 4,428 base pairs (GenBank Accession No. EU338534). The organization of the rRNA genes is LSU rRNA-ITS-SSU rRNA-IGS-5S, which corresponds with that of Nosema species closely related to Nosema bombycis. Phylogenetic analysis based on rRNA gene sequences show that this isolate is closely related to Nosema bombycis, Nosema plutellae, Nosema spodopterae, and Nosema antheraeae. The ultrastructure of all developmental stages of this microsporidium confirmed its placement in the genus Nosema. The isolate was successfully propagated in cell lines IPLB-LD652Y (Lymantria dispar) and NTU-LY (Lymantria xylina) and, in the in vitro system, it was frequently found to develop in the nuclei of the host cells, a circumstance that seldom occurs in other Nosema species. An extra-cellular vegetative stage of this microsporidium was also observed in the culture medium after 14 days of infection. The ECMDFs might be released from disrupted host cells.

Original language	English (US)
Pages (from-to)	85-93
Number of pages	9
Journal	Journal of Invertebrate Pathology
Volume	100
Issue number	2
DOIs	https://doi.org/10.1016/j.jip.2008.11.006
State	Published - Feb 2009
Externally published	Yes

Keywords

Extra-cellular microsporidian developmental forms
Intra-nuclear development
Microsporidium
Nosema
Phylogenetic analysis
RRNA

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics

Online availability

10.1016/j.jip.2008.11.006

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Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae). / Tsai, Yi Chun; Solter, Leellen F.; Wang, Chih Yuan et al.
In: Journal of Invertebrate Pathology, Vol. 100, No. 2, 02.2009, p. 85-93.

Research output: Contribution to journal › Article › peer-review

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title = "Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae)",

abstract = "A microsporidium possessing molecular and morphological characteristics of the genus Nosema was isolated from larvae of the thee-spot grass yellow butterfly, Eurema blanda arsakia. The complete rRNA gene sequences of the E. blanda isolate contained 4,428 base pairs (GenBank Accession No. EU338534). The organization of the rRNA genes is LSU rRNA-ITS-SSU rRNA-IGS-5S, which corresponds with that of Nosema species closely related to Nosema bombycis. Phylogenetic analysis based on rRNA gene sequences show that this isolate is closely related to Nosema bombycis, Nosema plutellae, Nosema spodopterae, and Nosema antheraeae. The ultrastructure of all developmental stages of this microsporidium confirmed its placement in the genus Nosema. The isolate was successfully propagated in cell lines IPLB-LD652Y (Lymantria dispar) and NTU-LY (Lymantria xylina) and, in the in vitro system, it was frequently found to develop in the nuclei of the host cells, a circumstance that seldom occurs in other Nosema species. An extra-cellular vegetative stage of this microsporidium was also observed in the culture medium after 14 days of infection. The ECMDFs might be released from disrupted host cells.",

keywords = "Extra-cellular microsporidian developmental forms, Intra-nuclear development, Microsporidium, Nosema, Phylogenetic analysis, RRNA",

author = "Tsai, {Yi Chun} and Solter, {Leellen F.} and Wang, {Chih Yuan} and Fan, {Huai Sheng} and Chang, {Chun che} and Wang, {Chung Hsiung}",

note = "Funding Information: This study was supported in part by the National Science Council, Republic of China, the Council of Agriculture, Executive Yuan, Taiwan, and the Illinois Natural History Survey. ",

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AU - Solter, Leellen F.

AU - Wang, Chih Yuan

AU - Fan, Huai Sheng

AU - Chang, Chun che

AU - Wang, Chung Hsiung

N1 - Funding Information: This study was supported in part by the National Science Council, Republic of China, the Council of Agriculture, Executive Yuan, Taiwan, and the Illinois Natural History Survey.

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Morphological and molecular studies of a microsporidium (Nosema sp.) isolated from the thee spot grass yellow butterfly, Eurema blanda arsakia (Lepidoptera: Pieridae)

Abstract

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