Morphogenesis and adhesion of Aureobasidium pullulans

J. H. Andrews, R. F. Harris, R. N. Spear, G. W. Lau, E. V. Nordheim

Research output: Contribution to journalArticle

Abstract

Two strains of the dimorphic fungus Aureobasidium pullulans were grown in liquid and on solid media varying in carbon and nitrogen content, and on leaf surfaces. Hyphae were observed in all systems but comprised a very low proportion (often below quantitative detection) of the total biomass. In liquid media, hyphae were found sparsely and only in the wash-zone on walls of the culture flasks. Yeast phase growth (blastospores) occurred in pH- buffered media that were nutrient balanced, or continuously carbon-limited (fed-batch culture), or carbon-exhausted (batch culture). Blastospores exposed to conditions with limited nitrogen but sufficient organic carbon, or to acidified media, converted to swollen cells and chlamydospores. The latter morphotypes accumulated carbon internally as lipid granules, and then externally as capsular and soluble extracellular polysaccharide. They were cohesive and also adhered more strongly to cellulose membranes overlying agar media or to leaves than did blastospores. Pullulanase treatment diminished the capsules, cohesion, and adhesion. Addition of soluble extracellular polysaccharide to blastospores enhanced their adhesion to leaves. We conclude that extracellular polysaccharide can play a role in adhesion of A. pullulans.

Original languageEnglish (US)
Pages (from-to)6-17
Number of pages12
JournalCanadian journal of microbiology
Volume40
Issue number1
DOIs
StatePublished - Jan 1 1994

Keywords

  • cell attachment
  • epiphytic growth
  • extracellular polysaccharide
  • leaf surface
  • phylloplane

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Genetics

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  • Cite this

    Andrews, J. H., Harris, R. F., Spear, R. N., Lau, G. W., & Nordheim, E. V. (1994). Morphogenesis and adhesion of Aureobasidium pullulans. Canadian journal of microbiology, 40(1), 6-17. https://doi.org/10.1139/m94-002