Monoclonal immunoglobulin a prevents adherence and invasion of polarized epithelial cell monolayers by Salmonella typhimurium

Pierre Michetti, Nadine Porta, Michael J. Mahan, James M. Slauch, John J. Mekalanos, AndréL L. Blum, Jean Pierre Kraehenbuhl, Marian R. Neutra

Research output: Contribution to journalArticle

Abstract

Background/Aims: Invasion of the intestinal epithelium is considered a critical step in Salmonella pathogenesis. Infection by Salmonella of cultured monolayers of polarized Madin-Darby canine kidney (MDCK) cells has been established as a simple in vitro system that mimics the invasion of intestinal enterocytes in vivo. This study analyzes the protective role of secretory immunoglobulin (Ig) A antibodies against epithelial invasion. Methods: Salmonella typhimurium was applied to MDCK cell monolayers in the presence or absence of a monoclonal, polymeric IgA antibody (Sal4) directed against an antigenic determinant exposed on the surface of wild-type S. typhimurium. Results: In the presence of Sal4 IgA, confluent monolayers of MDCK cells were protected against apical invasion by wild-type S.typhlmurium but not against a mutant strain that lacks the Sal4 epitope. Protection was Sal4-specific, dependent on the concentration of Sal4 in the apical medium, and occurred at IgA concentrations at which agglutination of IgA-bacterial complexes was observed. When MDCK cell monolayers were formaldehyde-fixed before incubation with Salmonella to prevent bacterial invasion, adhesion of Salmonella occurred in the absence of IgA and in the presence of control IgA but not in the presence of Sal4 IgA.Conclusions: IgA alone can prevent bacterial adherence and invasion of epithelial cells in the absence of other immune or nonimmune protective mechanisms.

Original languageEnglish (US)
Pages (from-to)915-923
Number of pages9
JournalGastroenterology
Volume107
Issue number4
DOIs
StatePublished - Oct 1994
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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    Michetti, P., Porta, N., Mahan, M. J., Slauch, J. M., Mekalanos, J. J., Blum, A. L., Kraehenbuhl, J. P., & Neutra, M. R. (1994). Monoclonal immunoglobulin a prevents adherence and invasion of polarized epithelial cell monolayers by Salmonella typhimurium. Gastroenterology, 107(4), 915-923. https://doi.org/10.1016/0016-5085(94)90214-3