Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of Staphylococcus aureus

Anand Subramanian; Joseph Irudayaraj; Thomas Ryan

doi:10.1016/j.snb.2005.04.030

Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of Staphylococcus aureus

Anand Subramanian, Joseph Irudayaraj, Thomas Ryan

Research output: Contribution to journal › Article › peer-review

Abstract

The capability of alkane monothiol and dithiol dendritic tether based self-assembled monolayers (SAM) in conjunction with surface plasmon resonance (SPR) for the detection of Staphylococcus aureus was investigated. An antibody based detection protocol for S. aureus, suspended in phosphate buffered saline with Tween 20 (PBST) was developed. Direct and sandwich assays were carried out on the immobilized antibodies against S. aureus. The effect of primary and secondary antibody concentrations on detection was investigated using a Box-Behnken surface response model. Specificity of anti-S. aureus to detect S. aureus in a mixture with Escherichia coli O157:H7 was also researched. Both the surfaces could detect S. aureus to the level of 10 ⁵ CFU/ml in a sandwich assay, with good specificity against E. coli O157:H7. Results indicate that mono and dithiol self-assembled monolayers can be employed for rapid detection of S. aureus.

Original language	English (US)
Pages (from-to)	192-198
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	114
Issue number	1
DOIs	https://doi.org/10.1016/j.snb.2005.04.030
State	Published - Mar 30 2006
Externally published	Yes

Keywords

Dithiols
Monothiols
Pathogen detection
Self-assembled monolayers
Staphylococcus aureus
Surface plasmon resonance

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Instrumentation
Condensed Matter Physics
Surfaces, Coatings and Films
Metals and Alloys
Electrical and Electronic Engineering
Materials Chemistry

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10.1016/j.snb.2005.04.030

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title = "Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of Staphylococcus aureus",

abstract = "The capability of alkane monothiol and dithiol dendritic tether based self-assembled monolayers (SAM) in conjunction with surface plasmon resonance (SPR) for the detection of Staphylococcus aureus was investigated. An antibody based detection protocol for S. aureus, suspended in phosphate buffered saline with Tween 20 (PBST) was developed. Direct and sandwich assays were carried out on the immobilized antibodies against S. aureus. The effect of primary and secondary antibody concentrations on detection was investigated using a Box-Behnken surface response model. Specificity of anti-S. aureus to detect S. aureus in a mixture with Escherichia coli O157:H7 was also researched. Both the surfaces could detect S. aureus to the level of 10 5 CFU/ml in a sandwich assay, with good specificity against E. coli O157:H7. Results indicate that mono and dithiol self-assembled monolayers can be employed for rapid detection of S. aureus.",

keywords = "Dithiols, Monothiols, Pathogen detection, Self-assembled monolayers, Staphylococcus aureus, Surface plasmon resonance",

author = "Anand Subramanian and Joseph Irudayaraj and Thomas Ryan",

note = "Funding Information: The authors acknowledge the USDA Milk Safety grant and NASA Food Technology Commercial Space Center (Iowa State University) for funding this work. We thank Dr. Chitrita Debroy (Gastroenteric Disease Center, The Pennsylvania State University, University Park, PA 16802) for kindly providing the bacterial cultures. Ann Flemmings at the Corporate Foundation Relations at The Pennsylvania State University, University Park, PA is acknowledged for her help in industry relations. ",

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AU - Ryan, Thomas

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PY - 2006/3/30

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N2 - The capability of alkane monothiol and dithiol dendritic tether based self-assembled monolayers (SAM) in conjunction with surface plasmon resonance (SPR) for the detection of Staphylococcus aureus was investigated. An antibody based detection protocol for S. aureus, suspended in phosphate buffered saline with Tween 20 (PBST) was developed. Direct and sandwich assays were carried out on the immobilized antibodies against S. aureus. The effect of primary and secondary antibody concentrations on detection was investigated using a Box-Behnken surface response model. Specificity of anti-S. aureus to detect S. aureus in a mixture with Escherichia coli O157:H7 was also researched. Both the surfaces could detect S. aureus to the level of 10 5 CFU/ml in a sandwich assay, with good specificity against E. coli O157:H7. Results indicate that mono and dithiol self-assembled monolayers can be employed for rapid detection of S. aureus.

AB - The capability of alkane monothiol and dithiol dendritic tether based self-assembled monolayers (SAM) in conjunction with surface plasmon resonance (SPR) for the detection of Staphylococcus aureus was investigated. An antibody based detection protocol for S. aureus, suspended in phosphate buffered saline with Tween 20 (PBST) was developed. Direct and sandwich assays were carried out on the immobilized antibodies against S. aureus. The effect of primary and secondary antibody concentrations on detection was investigated using a Box-Behnken surface response model. Specificity of anti-S. aureus to detect S. aureus in a mixture with Escherichia coli O157:H7 was also researched. Both the surfaces could detect S. aureus to the level of 10 5 CFU/ml in a sandwich assay, with good specificity against E. coli O157:H7. Results indicate that mono and dithiol self-assembled monolayers can be employed for rapid detection of S. aureus.

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KW - Self-assembled monolayers

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KW - Surface plasmon resonance

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Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of Staphylococcus aureus

Abstract

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