Mono and dithiol surfaces on surface plasmon resonance biosensors for detection of Staphylococcus aureus

Anand Subramanian, Joseph Irudayaraj, Thomas Ryan

Research output: Contribution to journalArticle

Abstract

The capability of alkane monothiol and dithiol dendritic tether based self-assembled monolayers (SAM) in conjunction with surface plasmon resonance (SPR) for the detection of Staphylococcus aureus was investigated. An antibody based detection protocol for S. aureus, suspended in phosphate buffered saline with Tween 20 (PBST) was developed. Direct and sandwich assays were carried out on the immobilized antibodies against S. aureus. The effect of primary and secondary antibody concentrations on detection was investigated using a Box-Behnken surface response model. Specificity of anti-S. aureus to detect S. aureus in a mixture with Escherichia coli O157:H7 was also researched. Both the surfaces could detect S. aureus to the level of 10 5 CFU/ml in a sandwich assay, with good specificity against E. coli O157:H7. Results indicate that mono and dithiol self-assembled monolayers can be employed for rapid detection of S. aureus.

Original languageEnglish (US)
Pages (from-to)192-198
Number of pages7
JournalSensors and Actuators, B: Chemical
Volume114
Issue number1
DOIs
StatePublished - Mar 30 2006
Externally publishedYes

Keywords

  • Dithiols
  • Monothiols
  • Pathogen detection
  • Self-assembled monolayers
  • Staphylococcus aureus
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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