Monitoring reactivation of latent HIV by label-free gradient light interference microscopy

Neha Goswami, Yiyang Lu, Mikhail E. Kandel, Michael J. Fanous, Kathrin Bohn-Wippert, Erin N. Tevonian, Roy D. Dar, Gabriel Popescu

Research output: Contribution to journalArticlepeer-review


Human immunodeficiency virus (HIV) can infect cells and take a quiescent and nonexpressive state called latency. In this study, we report insights provided by label-free, gradient light interference microscopy (GLIM) about the changes in dry mass, diameter, and dry mass density associated with infected cells that occur upon reactivation. We discovered that the mean cell dry mass and mean diameter of latently infected cells treated with reactivating drug, TNF-α, are higher for latent cells that reactivate than those of the cells that did not reactivate. Cells with mean dry mass and diameter less than approximately 10 pg and 8 μm, respectively, remain exclusively in the latent state. Also, cells with mean dry mass greater than approximately 28-30 pg and mean diameter greater than 11–12 μm have a higher probability of reactivating. This study is significant as it presents a new label-free approach to quantify latent reactivation of a virus in single cells.

Original languageEnglish (US)
Article number102940
Issue number8
StatePublished - Aug 20 2021


  • immunology
  • optics
  • virology

ASJC Scopus subject areas

  • General


Dive into the research topics of 'Monitoring reactivation of latent HIV by label-free gradient light interference microscopy'. Together they form a unique fingerprint.

Cite this