@article{c98a268b798d412bacd46262235906f5,
title = "Monitoring focal adhesion kinase phosphorylation dynamics in live cells",
abstract = "Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.",
author = "Damayanti, \{Nur P.\} and Kevin Buno and Nagarajan Narayanan and \{Voytik Harbin\}, \{Sherry L.\} and Meng Deng and Irudayaraj, \{Joseph M.K.\}",
note = "The authors acknowledge the grant from W. M. Keck Foundation to JI. This research was supported in part by the grant R01HL10962 (S. L. V.-H. and M. C. Y.) from the National Heart, Lung, and Blood Institute as well as the Incentive Grant funds provided by the Purdue University, Office of the Executive Vice President for Research and Partnerships (S. L. V.-H.). Partial funding from the Toxicology Pillars grant (EVPRP, Purdue University) and the USDA Cooperative Agreement OSQR (8072-42000-077), Eastern Regional Research Center to JI is appreciated. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Heart, Lung, and Blood Institute or the National Institutes of Health. K. P. B. is a recipient of an NSF Graduate Fellowship (DGE-1333468).",
year = "2017",
month = aug,
day = "7",
doi = "10.1039/c7an00471k",
language = "English (US)",
volume = "142",
pages = "2713--2716",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "15",
}