Abstract
Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 2713-2716 |
| Number of pages | 4 |
| Journal | Analyst |
| Volume | 142 |
| Issue number | 15 |
| DOIs | |
| State | Published - Aug 7 2017 |
| Externally published | Yes |
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Environmental Chemistry
- Spectroscopy
- Electrochemistry