Monitoring focal adhesion kinase phosphorylation dynamics in live cells

Nur P. Damayanti, Kevin Buno, Nagarajan Narayanan, Sherry L. Voytik Harbin, Meng Deng, Joseph M.K. Irudayaraj

Research output: Contribution to journalArticlepeer-review

Abstract

Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.

Original languageEnglish (US)
Pages (from-to)2713-2716
Number of pages4
JournalAnalyst
Volume142
Issue number15
DOIs
StatePublished - Aug 7 2017
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

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