Monitoring focal adhesion kinase phosphorylation dynamics in live cells

Nur P. Damayanti, Kevin Buno, Nagarajan Narayanan, Sherry L. Voytik Harbin, Meng Deng, Joseph Maria Kumar Irudayaraj

Research output: Contribution to journalArticle

Abstract

Focal adhesion kinase (FAK) is a cytoplasmic non-receptor tyrosine kinase essential for a diverse set of cellular functions. Current methods for monitoring FAK activity in response to an extracellular stimulus lack spatiotemporal resolution and/or the ability to perform multiplex detection. Here we report on a novel approach to monitor the real-time kinase phosphorylation activity of FAK in live single cells by fluorescence lifetime imaging.

Original languageEnglish (US)
Pages (from-to)2713-2716
Number of pages4
JournalAnalyst
Volume142
Issue number15
DOIs
StatePublished - Aug 7 2017
Externally publishedYes

Fingerprint

Focal Adhesion Protein-Tyrosine Kinases
Phosphorylation
adhesion
Adhesion
Monitoring
monitoring
Optical Imaging
Cytoplasmic and Nuclear Receptors
Protein-Tyrosine Kinases
fluorescence
Phosphotransferases
Fluorescence
Imaging techniques

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Damayanti, N. P., Buno, K., Narayanan, N., Voytik Harbin, S. L., Deng, M., & Irudayaraj, J. M. K. (2017). Monitoring focal adhesion kinase phosphorylation dynamics in live cells. Analyst, 142(15), 2713-2716. https://doi.org/10.1039/c7an00471k

Monitoring focal adhesion kinase phosphorylation dynamics in live cells. / Damayanti, Nur P.; Buno, Kevin; Narayanan, Nagarajan; Voytik Harbin, Sherry L.; Deng, Meng; Irudayaraj, Joseph Maria Kumar.

In: Analyst, Vol. 142, No. 15, 07.08.2017, p. 2713-2716.

Research output: Contribution to journalArticle

Damayanti, NP, Buno, K, Narayanan, N, Voytik Harbin, SL, Deng, M & Irudayaraj, JMK 2017, 'Monitoring focal adhesion kinase phosphorylation dynamics in live cells', Analyst, vol. 142, no. 15, pp. 2713-2716. https://doi.org/10.1039/c7an00471k
Damayanti NP, Buno K, Narayanan N, Voytik Harbin SL, Deng M, Irudayaraj JMK. Monitoring focal adhesion kinase phosphorylation dynamics in live cells. Analyst. 2017 Aug 7;142(15):2713-2716. https://doi.org/10.1039/c7an00471k
Damayanti, Nur P. ; Buno, Kevin ; Narayanan, Nagarajan ; Voytik Harbin, Sherry L. ; Deng, Meng ; Irudayaraj, Joseph Maria Kumar. / Monitoring focal adhesion kinase phosphorylation dynamics in live cells. In: Analyst. 2017 ; Vol. 142, No. 15. pp. 2713-2716.
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