Molybdopterin biosynthesis: Trapping an unusual purine ribose adduct in the MoaA-catalyzed reaction

Angad P. Mehta, Sameh H. Abdelwahed, Tadhg P. Begley

Research output: Contribution to journalArticlepeer-review

Abstract

MoaA/MoaC catalyze a remarkable rearrangement reaction in which guanosine-5′-triphosphate (GTP) is converted to cyclic pyranopterin monophosphate (cPMP). In this reaction, the C8 of GTP is inserted between the C2′ and the C3′ carbons of the GTP ribose. Previous experiments with GTP isotopomers demonstrated that the ribose C3′ hydrogen atom is abstracted by the adenosyl radical. This led to a novel mechanistic proposal involving an intermediate with a bond between the C8 of guanine and C3′ of the ribose. This paper describes the use of 2′,3′-dideoxyGTP to trap this intermediate.

Original languageEnglish (US)
Pages (from-to)10883-10885
Number of pages3
JournalJournal of the American Chemical Society
Volume135
Issue number30
DOIs
StatePublished - Jul 31 2013
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

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