Molecular taxonomy using single‐strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes

R. H. Hiss, D. E. Norris, C. H. Dietrich, R. F. Whitcomb, D. F. West, C. F. Bosio, S. Kambhampati, J. Piesman, M. F. Antolin, W. C.Black IV

Research output: Contribution to journalArticle

Abstract

Single‐strand conformation polymorphism (SSCP) analysis detects single point mutations in DNA molecules. We demonstrate that SSCP analysis of mitochondrial ribosomal DNA (rDNA) genes is a sensitive taxonomic tool because these genes often differ at numerous sites among closely related species. Using conserved primers, portions of the 12S or 16S rDNA genes were amplified using the polymerase chain reaction (PCR) in congeneric species of ticks, leaf hoppers, mosquitoes, and closely related endoparasitic wasps. SSCP was performed and products were visualized with silver staining. Species‐specific patterns were observed in all taxa. Intraspecific variation at the level of single nucleotide substitutions was detected. SSCP diagnostics are less expensive and time consuming to develop than PCR with species‐specific primers, and, unlike PCR with arbitrary primers, there is minimal concern with DNA contamination from non‐target organisms.

Original languageEnglish (US)
Pages (from-to)171-182
Number of pages12
JournalInsect Molecular Biology
Volume3
Issue number3
DOIs
StatePublished - Aug 1994
Externally publishedYes

Keywords

  • PCR
  • SSCP
  • mitochondrial DNA
  • molecular taxonomy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Insect Science

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    Hiss, R. H., Norris, D. E., Dietrich, C. H., Whitcomb, R. F., West, D. F., Bosio, C. F., Kambhampati, S., Piesman, J., Antolin, M. F., & IV, W. C. B. (1994). Molecular taxonomy using single‐strand conformation polymorphism (SSCP) analysis of mitochondrial ribosomal DNA genes. Insect Molecular Biology, 3(3), 171-182. https://doi.org/10.1111/j.1365-2583.1994.tb00164.x