Molecular motors one at a time: FIONA to the rescue

Comert Kural, Hamza Balci, Paul R. Selvin

Research output: Contribution to journalArticle

Abstract

Processive molecular motors act as intracellular transporters of a broad range of cargoes varying from organelles to messenger RNAs. Due to the nanometre range movements and complex dynamics of these motors, highly specialized tools are required to study them, in particular at the single-molecule level. Such tools are what physicists are providing for understanding these biological systems. Fluorescence based real-time localization techniques, with 1 nm spatial resolution and down to 1 ms temporal resolution (FIONA: fluorescence imaging with one-nanometre accuracy), and their applications to a group of molecular motors (myosin V, myosin VI, kinesin, and dynein) are the topics of this paper. In addition to the well established in vitro studies, the recent applications of these techniques to the much more challenging, but also more informative, in vivo realm will be discussed.

Original languageEnglish (US)
Pages (from-to)S3979-S3995
JournalJournal of Physics Condensed Matter
Volume17
Issue number47
DOIs
StatePublished - Nov 30 2005

Fingerprint

myosins
Myosin Type V
Fluorescence
ribonucleic acids
Dyneins
transporter
Kinesin
fluorescence
organelles
cargo
Biological systems
temporal resolution
spatial resolution
Imaging techniques
Messenger RNA
Molecules
molecules
myosin VI

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics

Cite this

Molecular motors one at a time : FIONA to the rescue. / Kural, Comert; Balci, Hamza; Selvin, Paul R.

In: Journal of Physics Condensed Matter, Vol. 17, No. 47, 30.11.2005, p. S3979-S3995.

Research output: Contribution to journalArticle

Kural, Comert ; Balci, Hamza ; Selvin, Paul R. / Molecular motors one at a time : FIONA to the rescue. In: Journal of Physics Condensed Matter. 2005 ; Vol. 17, No. 47. pp. S3979-S3995.
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