Molecular identification of the D-genome chromosomes of wheat

A. Lane Rayburn, B. S. Gill

Research output: Contribution to journalArticlepeer-review


A highly repeated DNA sequence isolated from Aegllops squarrosa was used to Identify specific chromosomes in hexaploid wheat. The DNA sequence found In clone pAS1 appears D-genome specific and, therefore, allows for the unambiguous identification of the D-genome chromosomes. In situ hybridization using blotin labeled DNA probes was found to be a rapid and reliable technique to identify sites of hybridization on D-genome chromosomes. Multiple sites of hybridization of the sequence allowed the Identification of all seven pairs of the D-genome chromosomes.

Original languageEnglish (US)
Pages (from-to)253-255
Number of pages3
JournalJournal of Heredity
Issue number4
StatePublished - Jul 1986
Externally publishedYes

ASJC Scopus subject areas

  • General Medicine


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