Molecular identification of the D-genome chromosomes of wheat

A Lane Rayburn, B. S. Gill

Research output: Contribution to journalArticle

Abstract

A highly repeated DNA sequence isolated from Aegllops squarrosa was used to Identify specific chromosomes in hexaploid wheat. The DNA sequence found In clone pAS1 appears D-genome specific and, therefore, allows for the unambiguous identification of the D-genome chromosomes. In situ hybridization using blotin labeled DNA probes was found to be a rapid and reliable technique to identify sites of hybridization on D-genome chromosomes. Multiple sites of hybridization of the sequence allowed the Identification of all seven pairs of the D-genome chromosomes.

Original languageEnglish (US)
Pages (from-to)253-255
Number of pages3
JournalJournal of Heredity
Volume77
Issue number4
DOIs
StatePublished - Jan 1 1986
Externally publishedYes

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Chromosomes, Human, 13-15
Triticum
Genome
DNA Probes
In Situ Hybridization
Clone Cells
Chromosomes

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Molecular identification of the D-genome chromosomes of wheat. / Rayburn, A Lane; Gill, B. S.

In: Journal of Heredity, Vol. 77, No. 4, 01.01.1986, p. 253-255.

Research output: Contribution to journalArticle

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