Abstract

A rapid label-free approach for molecular histopathology is presented and reviewed. Broadband vibrational spectra are generated by nonlinear interferometric vibrational imaging (NIVI), a coherent anti-Stokes Raman scattering (CARS)-based technique that uses interferometry and signal processing approaches to acquire Raman-like profiles with suppression of the non-resonant background. This allows for the generation of images that provide contrast based on quantitative chemical composition with high spatial and spectral resolution. Algorithms are demonstrated for reducing the diagnostic spectral information into color-coded composite images for the rapid identification of chemical constituents in skin, as well as differentiating normal from abnormal tissue in a pre-clinical tumor model for human breast cancer. This technology and methodology could result in an alternative method to the traditional histological staining and subjective interpretation procedure currently used in the diagnosis of disease, and has the potential for future in vivo molecular histopathology.

Original languageEnglish (US)
Title of host publicationEuropean Conference on Biomedical Optics, ECBO 2011
StatePublished - Dec 1 2011
EventEuropean Conference on Biomedical Optics, ECBO 2011 - Munich, Germany
Duration: May 22 2011May 26 2011

Publication series

NameOptics InfoBase Conference Papers
ISSN (Electronic)2162-2701

Other

OtherEuropean Conference on Biomedical Optics, ECBO 2011
CountryGermany
CityMunich
Period5/22/115/26/11

Keywords

  • Cancer
  • Coherence
  • Coherent anti-stokes raman scattering
  • Histology
  • Nonlinear interferometric vibrational imaging

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Boppart, S. A. (2011). Molecular histopathology by nonlinear interferometric vibrational imaging. In European Conference on Biomedical Optics, ECBO 2011 [808704] (Optics InfoBase Conference Papers).