Molecular dissection and improvement of the nodule symbiosis in legumes

Research output: Contribution to journalArticlepeer-review

Abstract

The initiation and development of nitrogen (N2) fixing nodules in the roots of leguminous plants occurs by the induction of cell division and redifferentiation in the root cortex, followed by the formation of a meristem and progressive differentiation of specialized cells and tissues. During this process, competent rhizobia invade the root and become specialized N2-fixing endosymbionts. The onset of the symbiosis is largely mediated by an exchange of diffusible signals, bacterial lipo-oligosaccharides being the main determinants of specificity and the initial inducers of plant responses. It is however the host which controls most facets of the nodulation process, including nodule morphology, efficiency, specificity and function. The dissection of plant mechanisms underlying signal-transduction during nodulation may be crucial to understand and then manipulate the symbiosis. Positional cloning or gene targeting offer strategies that promise the identification of crucial plant genes determining nodulation. The search for the nts-1 gene that controls nodulation in soybean illustrates the challenges and limitations of positional cloning. It also shows how biotechnology can offer tools to help in the breeding of plant traits important to agriculture. Molecular dissection of the symbiosis will ultimately be used to improve N2 fixation by molecular breeding and genetic engineering in legumes.

Original languageEnglish (US)
Pages (from-to)47-68
Number of pages22
JournalField Crops Research
Volume53
Issue number1-3
DOIs
StatePublished - Jul 1997
Externally publishedYes

Keywords

  • Autoregulation
  • Legumes
  • Nitrogen fixation
  • Nodulation
  • Positional cloning
  • Soybean
  • Symbiosis genes

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Fingerprint Dive into the research topics of 'Molecular dissection and improvement of the nodule symbiosis in legumes'. Together they form a unique fingerprint.

Cite this