Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Yating Liu; Wen Xie; Xin Yang; Litao Guo; Shaoli Wang; Qingjun Wu; Zezhong Yang; Xuguo Zhou; Youjun Zhang

doi:10.1016/j.gene.2016.01.005

Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Yating Liu, Wen Xie, Xin Yang, Litao Guo, Shaoli Wang, Qingjun Wu, Zezhong Yang, Xuguo Zhou, Youjun Zhang

Research output: Contribution to journal › Article › peer-review

Abstract

The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

Original language	English (US)
Pages (from-to)	104-110
Number of pages	7
Journal	Gene
Volume	580
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2016.01.005
State	Published - Apr 15 2016
Externally published	Yes

Keywords

Alternative splicing
Bemisia tabaci
Haplodiploidy
PSI
Sex determination

ASJC Scopus subject areas

Genetics

Online availability

10.1016/j.gene.2016.01.005

Library availability

Discover UIUC Full Text

Cite this

@article{0f78c7fdf0fd4ff8964c0096fcd0cc2a,

title = "Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties",

abstract = "The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.",

keywords = "Alternative splicing, Bemisia tabaci, Haplodiploidy, PSI, Sex determination",

author = "Yating Liu and Wen Xie and Xin Yang and Litao Guo and Shaoli Wang and Qingjun Wu and Zezhong Yang and Xuguo Zhou and Youjun Zhang",

note = "Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

month = apr,

day = "15",

doi = "10.1016/j.gene.2016.01.005",

language = "English (US)",

volume = "580",

pages = "104--110",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier B.V.",

number = "2",

}

TY - JOUR

T1 - Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

AU - Liu, Yating

AU - Xie, Wen

AU - Yang, Xin

AU - Guo, Litao

AU - Wang, Shaoli

AU - Wu, Qingjun

AU - Yang, Zezhong

AU - Zhou, Xuguo

AU - Zhang, Youjun

PY - 2016/4/15

Y1 - 2016/4/15

N2 - The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

AB - The P-element somatic inhibitor (PSI) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in Bombyx mori. In this study, we identified and characterized a PSI homolog in Bemisia tabaci (BtPSI). BtPSI cDNA had a total length of 5700 bp and contained a predicted open reading frame (ORF) of 2208 nucleotides encoding for 735 amino acids. Multiple sequence alignments of the common regions of PSI proteins from B. tabaci and five other insect species revealed a high degree of sequence conservation. BtPSI is expressed in all stages of B. tabaci development, and expression did not significantly differ between female and male adult. A total of 92 BtPSI isoforms (78 in female and 22 in male) were identified, and a marker indicating the female-specific form was found. These results increase the understanding of genes that may determine sex in B. tabaci and provide a foundation for research on the sex determination mechanism in this insect.

KW - Alternative splicing

KW - Bemisia tabaci

KW - Haplodiploidy

KW - PSI

KW - Sex determination

UR - http://www.scopus.com/inward/record.url?scp=84957437509&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957437509&partnerID=8YFLogxK

U2 - 10.1016/j.gene.2016.01.005

DO - 10.1016/j.gene.2016.01.005

M3 - Article

C2 - 26773355

AN - SCOPUS:84957437509

SN - 0378-1119

VL - 580

SP - 104

EP - 110

JO - Gene

JF - Gene

IS - 2

ER -

Molecular cloning of the sex-related gene PSI in Bemisia tabaci and its alternative splicing properties

Abstract

Keywords

ASJC Scopus subject areas

Online availability

Library availability

Related links

Fingerprint

Cite this