Molecular characterization of Fusarium head blight resistance in Wangshuibai with simple sequence repeat and amplified fragment length polymorphism markers

Wenchun Zhou, Frederic L. Kolb, Jianbin Yu, Guihua Bai, Larry K. Boze, Leslie L. Domier

Research output: Contribution to journalArticlepeer-review

Abstract

Molecular mapping of Fusarium head blight (FHB) resistance quantitative trait loci (QTL) and marker-assisted selection of these QTL will aid in the development of resistant cultivars. Most reported FHB resistance QTL are from 'Sumai 3' and its derivatives. 'Wangshuibai' is a FHB-resistant landrace that originated from China and is not known to be related to 'Sumai 3'. A mapping population of 139 F5:6 recombinant inbred lines was developed from a cross of 'Wangshuibai' and 'Wheaton'. This population was developed to map the FHB-resistant QTL in 'Wangshuibai' and was evaluated twice for Type II FHB resistance. A total of 1196 simple sequence repeat and amplified fragment length polymorphism markers were screened on this population, and four FHB resistance QTL were detected. A major QTL near the end of 3BS explained 37.3% of the phenotypic variation. Another QTL on 3BS, located close to the centromere, explained 7.4% of the phenotypic variation. Two additional QTL on 7AL and 1BL explained 9.8% and 11.9% of the phenotypic variation, respectively. The simple sequence repeat and amplified fragment length polymorphism markers closely linked to these QTL may be useful for stacking QTL from 'Wangshuibai' and other sources to develop cultivars with transgressive FHB resistance.

Original languageEnglish (US)
Pages (from-to)1137-1143
Number of pages7
JournalGenome
Volume47
Issue number6
DOIs
StatePublished - Dec 2004

Keywords

  • Head scab
  • Marker-assisted selection
  • Molecular mapping
  • Triticum aestivum L.
  • Wheat

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics

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