Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

Tuba Yasmin; Berlin D. Nelson; Houston A. Hobbs; Nancy K. McCoppin; Kris N. Lambert; Leslie L. Domier

doi:10.1007/s00705-016-3152-9

Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

Tuba Yasmin, Berlin D. Nelson, Houston A. Hobbs, Nancy K. McCoppin, Kris N. Lambert, Leslie L. Domier

Crop Sciences

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Abstract

The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3’ nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.

Original language	English (US)
Pages (from-to)	1089-1092
Number of pages	4
Journal	Archives of Virology
Volume	162
Issue number	4
DOIs	https://doi.org/10.1007/s00705-016-3152-9
State	Published - Apr 1 2017

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Virology

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10.1007/s00705-016-3152-9

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title = "Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing",

abstract = "The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3{\textquoteright} nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.",

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AU - Yasmin, Tuba

AU - Nelson, Berlin D.

AU - Hobbs, Houston A.

AU - McCoppin, Nancy K.

AU - Lambert, Kris N.

AU - Domier, Leslie L.

PY - 2017/4/1

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AB - The complete nucleotide sequence of a new soybean-infecting member of the genus Nepovirus (provisionally named “soybean latent spherical virus” [SLSV]) was identified by high-throughput sequencing of RNAs from soybean leaf samples from North Dakota, USA. The sequences of RNAs 1 (8,190 nt) and 2 (5,788 nt) were completed by rapid amplification of cDNA ends. Each contained a single long open reading frame and a 3’ nontranslated region of greater than 1,500 nt. The predicted amino acid sequences of the two ORFs were most closely related to nepoviruses in subgroup C. Full-length cDNAs of RNAs 1 and 2 were cloned and used to inoculate soybean plants, which did not display obvious symptoms. These results suggest that SLSV represents a new species in the genus Nepovirus.

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Molecular characterization of a new soybean-infecting member of the genus Nepovirus identified by high-throughput sequencing

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