Molecular characterization and sex-related seasonal expression of thyroid receptor subtypes in goldfish

Erik Nelson, H. R. Habibi

Research output: Contribution to journalArticle

Abstract

The thyroid hormones, acting through the nuclear thyroid receptors (TRs), play important roles in the growth and development of vertebrates. The present study investigated the molecular structure and season-related expression of the TR isoforms in the male and female goldfish pituitary, brain, liver, gonads, gut, heart, and muscle. Based on sequence alignment with other species, the results demonstrate the presence of: (1) a TRα (TRα-1) consisting of 1496 nucleotides encoding a 466 amino acid protein, (2) a novel splice variant of TRα (TRα-2) containing an out-of-frame deletion of 246 nucleotides in the ligand-binding domain consisting of 1251 nucleotides encoding a 378 amino acid protein, (3) a novel transcript resembling TRα, except for non-homology in the hinge region and a premature stop codon prior to the ligand-binding domain (TRα-truncated; 1418 nucleotides, 206 amino acid protein), and (4) TRβ consisting of 1823 nucleotides encoding a 395 amino acid protein. The findings provide the first demonstration of the presence of a truncated TR isoform in non-mammalian vertebrates. In goldfish, the expression patterns for all TRs subtypes were found to be remarkably similar in both male and female, changing significantly before and during reproductive season. The results provide a frame work for better understanding of the functional significance of novel TR forms and TR subtypes in fish and other vertebrates.

Original languageEnglish (US)
Pages (from-to)83-95
Number of pages13
JournalMolecular and Cellular Endocrinology
Volume253
Issue number1-2
DOIs
StatePublished - Jul 11 2006
Externally publishedYes

Fingerprint

Goldfish
Thyroid Gland
Nucleotides
Amino Acids
Protein Isoforms
Proteins
Ligands
Hinges
Vertebrates
Thyroid Hormones
Liver
Fish
Molecular structure
Muscle
Brain
Demonstrations
Frameshift Mutation
Sequence Alignment
Nonsense Codon
Gonads

Keywords

  • Cloning
  • Nuclear receptors
  • Seasonality
  • Tissue expression

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Molecular characterization and sex-related seasonal expression of thyroid receptor subtypes in goldfish. / Nelson, Erik; Habibi, H. R.

In: Molecular and Cellular Endocrinology, Vol. 253, No. 1-2, 11.07.2006, p. 83-95.

Research output: Contribution to journalArticle

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