Molecular biology of biotin attachment to proteins

Anne Chapman-Smith, John E. Cronan

Research output: Contribution to journalArticlepeer-review


Enzymatic attachment of biotin to proteins requires the interaction of a distinct domain of the acceptor protein (the 'biotin domain') with the enzyme, biotin protein ligase, that catalyzes this essential and rare post- translational modification. Both biotin domains and biotin protein ligases are very strongly conserved throughout biology. This review concerns the protein structures and mechanisms involved in the covalent attachment of biotin to proteins.

Original languageEnglish (US)
Pages (from-to)477S-484S
JournalJournal of Nutrition
Issue number2 SUPPL.
StatePublished - 1999


  • Acceptor
  • Biotin
  • Domain
  • Ligase
  • Post-translational

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics


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