TY - JOUR
T1 - Molecular basis of fertilization in the mouse
AU - Miller, David J.
AU - Shur, Barry D.
PY - 1994/8
Y1 - 1994/8
N2 - Recent studies of mouse fertilization have identified two complementary gamete receptors that mediate sperm-egg binding. Sperm surface β1,4-galactosyltransferase (GalTase) binds to specific oligosaccharides of the egg coat (zona pellucida) glycoprotein ZP3. Evidence suggests that these same molecules may stimulate the acrosome reaction in sperm. After the acrosome reaction, it is thought that sperm remain adherent to the zona by binding another glycoprotein, ZP2. The acrosome-reacted sperm releases hydrolytic enzymes, including acrosin and N-acetylglucosaminidase, enabling it to penetrate the zona pellucida. After the penetrating sperm binds to the egg membrane and activates development, N-acetylglucosaminidase is exocytosed from egg cortical granules and, as part of the zona block to polyspermy, globally removes the sperm GalTase binding site from ZP3 oligosaccharides.
AB - Recent studies of mouse fertilization have identified two complementary gamete receptors that mediate sperm-egg binding. Sperm surface β1,4-galactosyltransferase (GalTase) binds to specific oligosaccharides of the egg coat (zona pellucida) glycoprotein ZP3. Evidence suggests that these same molecules may stimulate the acrosome reaction in sperm. After the acrosome reaction, it is thought that sperm remain adherent to the zona by binding another glycoprotein, ZP2. The acrosome-reacted sperm releases hydrolytic enzymes, including acrosin and N-acetylglucosaminidase, enabling it to penetrate the zona pellucida. After the penetrating sperm binds to the egg membrane and activates development, N-acetylglucosaminidase is exocytosed from egg cortical granules and, as part of the zona block to polyspermy, globally removes the sperm GalTase binding site from ZP3 oligosaccharides.
KW - sperm/egg/β1,4-galactosyltransferase/ZP3/N-acetylglucosaminidase
UR - http://www.scopus.com/inward/record.url?scp=0000287662&partnerID=8YFLogxK
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U2 - 10.1006/sedb.1994.1033
DO - 10.1006/sedb.1994.1033
M3 - Article
AN - SCOPUS:0000287662
SN - 1044-5781
VL - 5
SP - 255
EP - 264
JO - Seminars in Developmental Biology
JF - Seminars in Developmental Biology
IS - 4
ER -