Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide

Megan M. Mahoney; Agnes Y. Lee; Donna J. Brezinski-Caliguri; Kenneth M. Huttner

doi:10.1016/0014-5793(95)01390-3

Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide

Megan M. Mahoney, Agnes Y. Lee, Donna J. Brezinski-Caliguri, Kenneth M. Huttner

Research output: Contribution to journal › Article › peer-review

Abstract

Cathelin-related genes are characterized by the presence of a prepro sequence which is highly conserved both within and between species. 3′ RACE analysis on sheep bone marrow RNA, using a primer based on a conserved cathelin family coding region, demonstrated the presence of at least three ovine cathelin-related cDNAs. One of these encodes a novel prepropeptide with a predicted C-terminal cleavage product RGLRRLGRKIAHGVKKYGPTVLRIIRIAG. The chemically synthesized form of this 29 amino acid peptide is shown to be a thermostable, broad spectrum, bactericidal agent.

Original language	English (US)
Pages (from-to)	519-522
Number of pages	4
Journal	FEBS Letters
Volume	377
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01390-3
State	Published - Dec 27 1995
Externally published	Yes

Keywords

3′ RACE
Antimicrobial peptide
Cathelin
Ovine

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

Online availability

10.1016/0014-5793(95)01390-3

Library availability

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title = "Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide",

abstract = "Cathelin-related genes are characterized by the presence of a prepro sequence which is highly conserved both within and between species. 3′ RACE analysis on sheep bone marrow RNA, using a primer based on a conserved cathelin family coding region, demonstrated the presence of at least three ovine cathelin-related cDNAs. One of these encodes a novel prepropeptide with a predicted C-terminal cleavage product RGLRRLGRKIAHGVKKYGPTVLRIIRIAG. The chemically synthesized form of this 29 amino acid peptide is shown to be a thermostable, broad spectrum, bactericidal agent.",

keywords = "3′ RACE, Antimicrobial peptide, Cathelin, Ovine",

author = "Mahoney, {Megan M.} and Lee, {Agnes Y.} and Brezinski-Caliguri, {Donna J.} and Huttner, {Kenneth M.}",

note = "Funding Information: A~knowledgments. We thank Mark Cioffi for assistance in obtaining the sheep tissue, Richard Gallo for advice on isolating RNA from ruminant bone marrow, and Andre Ouellette for assistance in establishing the antimicrobial assays. This work was supported, in part, by a grant from the Massachusetts Thoracic Society, 76391.01.",

year = "1995",

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doi = "10.1016/0014-5793(95)01390-3",

language = "English (US)",

volume = "377",

pages = "519--522",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "John Wiley & Sons, Ltd.",

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T1 - Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide

AU - Mahoney, Megan M.

AU - Lee, Agnes Y.

AU - Brezinski-Caliguri, Donna J.

AU - Huttner, Kenneth M.

N1 - Funding Information: A~knowledgments. We thank Mark Cioffi for assistance in obtaining the sheep tissue, Richard Gallo for advice on isolating RNA from ruminant bone marrow, and Andre Ouellette for assistance in establishing the antimicrobial assays. This work was supported, in part, by a grant from the Massachusetts Thoracic Society, 76391.01.

PY - 1995/12/27

Y1 - 1995/12/27

N2 - Cathelin-related genes are characterized by the presence of a prepro sequence which is highly conserved both within and between species. 3′ RACE analysis on sheep bone marrow RNA, using a primer based on a conserved cathelin family coding region, demonstrated the presence of at least three ovine cathelin-related cDNAs. One of these encodes a novel prepropeptide with a predicted C-terminal cleavage product RGLRRLGRKIAHGVKKYGPTVLRIIRIAG. The chemically synthesized form of this 29 amino acid peptide is shown to be a thermostable, broad spectrum, bactericidal agent.

AB - Cathelin-related genes are characterized by the presence of a prepro sequence which is highly conserved both within and between species. 3′ RACE analysis on sheep bone marrow RNA, using a primer based on a conserved cathelin family coding region, demonstrated the presence of at least three ovine cathelin-related cDNAs. One of these encodes a novel prepropeptide with a predicted C-terminal cleavage product RGLRRLGRKIAHGVKKYGPTVLRIIRIAG. The chemically synthesized form of this 29 amino acid peptide is shown to be a thermostable, broad spectrum, bactericidal agent.

KW - 3′ RACE

KW - Antimicrobial peptide

KW - Cathelin

KW - Ovine

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VL - 377

SP - 519

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JF - FEBS Letters

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ER -

Molecular analysis of the sheep cathelin family reveals a novel antimicrobial peptide

Abstract

Keywords

ASJC Scopus subject areas

Online availability

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