Molecular analysis of the D-genome of the Triticeae

A. Lane Rayburn, B. S. Gill

Research output: Contribution to journalArticlepeer-review

Abstract

The chromosome of three tetraploid Aegilops L. species containing the D-genome were analyzed by in situ hybridization with a repeated DNA sequence clone pAS1 isolated from Aegilops squarrosa and observed to be D-genome specific. This sequence is found on all seven D-genome chromosome pairs of A. squarrosa and hexaploid wheat. Two distinct D-genome patterns were observed in the tetraploid species. The D-genome of A. cylindrica was similar to hexaploid wheat. Seven pairs of chromosomes having large amounts and numerous sites of the sequence were observed. Five chromosome pairs with fewer and smaller sites of the repetitive sequence were observed in the D-genomes of A. crassa and A. ventricosa. In addition to these major repeated sequence differences, chromosomal modifications appear to have occurred between T. aestivum and A. cylindrica and between A. crassa and A. ventricosa resulting in changes with respect to location of the sequence between the respective species. D-genome divergence with respect to pAS1 sequence appears to have occurred at least in two forms, one characterized by the changes in amount of repetitive sequence and the second by changes in location of the sequence.

Original languageEnglish (US)
Pages (from-to)385-388
Number of pages4
JournalTheoretical and Applied Genetics
Volume73
Issue number3
DOIs
StatePublished - Jan 1 1987
Externally publishedYes

Keywords

  • Aegilops
  • Biotin labeling
  • Chromosome evolution
  • In situ hybridization

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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