Molecular Analysis of Archaea, Bacteria and Eucarya Communities in the Rumen

Bryan A White, Isaac Cann, S. A. Kocherginskaya, R. I. Aminov, L. A. Thill, Roderick Ian Mackie, R. Onodera

Research output: Contribution to journalReview article

Abstract

If rumen bacteria can be manipulated to utilize nutrients (i.e., ammonia and plant cell wall carbohydrates) more completely and efficiently, the need for protein supplementation can be reduced or eliminated and the digestion of fiber in forage or agricultural residue-based diets could be enhanced. However, these approaches require a complete and accurate description of the rumen community, as well as methods for the rapid and accurate detection of microbial density, diversity, phylogeny, and gene expression. Molecular ecology techniques based on small subunit (SSU) rRNA sequences, nucleic acid probes and the polymerase chain reaction (PCR) can potentially provide a complete description of the microbial ecology of the rumen of ruminant animals. The development of these molecular tools will result in greater insights into community structure and activity of gut microbial ecosystems in relation to functional interactions between different bacteria, spatial and temporal relationships between different microorganisms and between microorganisms and feed particles. Molecular approaches based on SSU rRNA serve to evaluate the presence of specific sequences in the community and provide a link between knowledge obtained from pure cultures and the microbial populations they represent in the rumen. The successful development and application of these methods promises to provide opportunities to link distribution and identity of gastrointestinal microbes in their natural environment with their genetic potential and in situ activities. The use of approaches for assessing population dynamics as well as for assessing community functionality will result in an increased understanding and a complete description of the gastrointestinal communities of production animals fed under different dietary regimes, and lead to new strategies for improving animal growth.

Original languageEnglish (US)
Pages (from-to)129-138
Number of pages10
JournalAsian-Australasian Journal of Animal Sciences
Volume12
Issue number1
DOIs
StatePublished - Feb 1999

Fingerprint

Archaea
Rumen
Eukaryota
rumen
microbial ecology
Bacteria
microorganisms
bacteria
microbial detection
Ecology
ribosomal RNA
rumen bacteria
animal growth
protein supplements
Nucleic Acid Probes
intestinal microorganisms
application methods
rapid methods
nucleic acids
ruminants

Keywords

  • DGGE
  • DNA
  • Ecology
  • Molecular Analysis
  • RNA
  • Review
  • Rumen Microbes

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Engineering(all)

Cite this

Molecular Analysis of Archaea, Bacteria and Eucarya Communities in the Rumen. / White, Bryan A; Cann, Isaac; Kocherginskaya, S. A.; Aminov, R. I.; Thill, L. A.; Mackie, Roderick Ian; Onodera, R.

In: Asian-Australasian Journal of Animal Sciences, Vol. 12, No. 1, 02.1999, p. 129-138.

Research output: Contribution to journalReview article

White, Bryan A ; Cann, Isaac ; Kocherginskaya, S. A. ; Aminov, R. I. ; Thill, L. A. ; Mackie, Roderick Ian ; Onodera, R. / Molecular Analysis of Archaea, Bacteria and Eucarya Communities in the Rumen. In: Asian-Australasian Journal of Animal Sciences. 1999 ; Vol. 12, No. 1. pp. 129-138.
@article{8db55df8a7154174a35ab75f910c49d8,
title = "Molecular Analysis of Archaea, Bacteria and Eucarya Communities in the Rumen",
abstract = "If rumen bacteria can be manipulated to utilize nutrients (i.e., ammonia and plant cell wall carbohydrates) more completely and efficiently, the need for protein supplementation can be reduced or eliminated and the digestion of fiber in forage or agricultural residue-based diets could be enhanced. However, these approaches require a complete and accurate description of the rumen community, as well as methods for the rapid and accurate detection of microbial density, diversity, phylogeny, and gene expression. Molecular ecology techniques based on small subunit (SSU) rRNA sequences, nucleic acid probes and the polymerase chain reaction (PCR) can potentially provide a complete description of the microbial ecology of the rumen of ruminant animals. The development of these molecular tools will result in greater insights into community structure and activity of gut microbial ecosystems in relation to functional interactions between different bacteria, spatial and temporal relationships between different microorganisms and between microorganisms and feed particles. Molecular approaches based on SSU rRNA serve to evaluate the presence of specific sequences in the community and provide a link between knowledge obtained from pure cultures and the microbial populations they represent in the rumen. The successful development and application of these methods promises to provide opportunities to link distribution and identity of gastrointestinal microbes in their natural environment with their genetic potential and in situ activities. The use of approaches for assessing population dynamics as well as for assessing community functionality will result in an increased understanding and a complete description of the gastrointestinal communities of production animals fed under different dietary regimes, and lead to new strategies for improving animal growth.",
keywords = "DGGE, DNA, Ecology, Molecular Analysis, RNA, Review, Rumen Microbes",
author = "White, {Bryan A} and Isaac Cann and Kocherginskaya, {S. A.} and Aminov, {R. I.} and Thill, {L. A.} and Mackie, {Roderick Ian} and R. Onodera",
year = "1999",
month = "2",
doi = "10.5713/ajas.1999.129",
language = "English (US)",
volume = "12",
pages = "129--138",
journal = "Asian-Australasian Journal of Animal Sciences",
issn = "1011-2367",
publisher = "Asian-Australasian Association of Animal Production Societies",
number = "1",

}

TY - JOUR

T1 - Molecular Analysis of Archaea, Bacteria and Eucarya Communities in the Rumen

AU - White, Bryan A

AU - Cann, Isaac

AU - Kocherginskaya, S. A.

AU - Aminov, R. I.

AU - Thill, L. A.

AU - Mackie, Roderick Ian

AU - Onodera, R.

PY - 1999/2

Y1 - 1999/2

N2 - If rumen bacteria can be manipulated to utilize nutrients (i.e., ammonia and plant cell wall carbohydrates) more completely and efficiently, the need for protein supplementation can be reduced or eliminated and the digestion of fiber in forage or agricultural residue-based diets could be enhanced. However, these approaches require a complete and accurate description of the rumen community, as well as methods for the rapid and accurate detection of microbial density, diversity, phylogeny, and gene expression. Molecular ecology techniques based on small subunit (SSU) rRNA sequences, nucleic acid probes and the polymerase chain reaction (PCR) can potentially provide a complete description of the microbial ecology of the rumen of ruminant animals. The development of these molecular tools will result in greater insights into community structure and activity of gut microbial ecosystems in relation to functional interactions between different bacteria, spatial and temporal relationships between different microorganisms and between microorganisms and feed particles. Molecular approaches based on SSU rRNA serve to evaluate the presence of specific sequences in the community and provide a link between knowledge obtained from pure cultures and the microbial populations they represent in the rumen. The successful development and application of these methods promises to provide opportunities to link distribution and identity of gastrointestinal microbes in their natural environment with their genetic potential and in situ activities. The use of approaches for assessing population dynamics as well as for assessing community functionality will result in an increased understanding and a complete description of the gastrointestinal communities of production animals fed under different dietary regimes, and lead to new strategies for improving animal growth.

AB - If rumen bacteria can be manipulated to utilize nutrients (i.e., ammonia and plant cell wall carbohydrates) more completely and efficiently, the need for protein supplementation can be reduced or eliminated and the digestion of fiber in forage or agricultural residue-based diets could be enhanced. However, these approaches require a complete and accurate description of the rumen community, as well as methods for the rapid and accurate detection of microbial density, diversity, phylogeny, and gene expression. Molecular ecology techniques based on small subunit (SSU) rRNA sequences, nucleic acid probes and the polymerase chain reaction (PCR) can potentially provide a complete description of the microbial ecology of the rumen of ruminant animals. The development of these molecular tools will result in greater insights into community structure and activity of gut microbial ecosystems in relation to functional interactions between different bacteria, spatial and temporal relationships between different microorganisms and between microorganisms and feed particles. Molecular approaches based on SSU rRNA serve to evaluate the presence of specific sequences in the community and provide a link between knowledge obtained from pure cultures and the microbial populations they represent in the rumen. The successful development and application of these methods promises to provide opportunities to link distribution and identity of gastrointestinal microbes in their natural environment with their genetic potential and in situ activities. The use of approaches for assessing population dynamics as well as for assessing community functionality will result in an increased understanding and a complete description of the gastrointestinal communities of production animals fed under different dietary regimes, and lead to new strategies for improving animal growth.

KW - DGGE

KW - DNA

KW - Ecology

KW - Molecular Analysis

KW - RNA

KW - Review

KW - Rumen Microbes

UR - http://www.scopus.com/inward/record.url?scp=0033484721&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033484721&partnerID=8YFLogxK

U2 - 10.5713/ajas.1999.129

DO - 10.5713/ajas.1999.129

M3 - Review article

AN - SCOPUS:0033484721

VL - 12

SP - 129

EP - 138

JO - Asian-Australasian Journal of Animal Sciences

JF - Asian-Australasian Journal of Animal Sciences

SN - 1011-2367

IS - 1

ER -