Modulation of gene silencing by Cdc7p via H4 K16 acetylation and phosphorylation of chromatin assembly factor CAF-1 in saccharomyces cerevisiae

Tiffany J. Young, Yi Cui, Joseph Irudayaraj, Ann L. Kirchmaier

Research output: Contribution to journalArticle

Abstract

CAF-1 is an evolutionarily conserved H3/H4 histone chaperone that plays a key role in replication-coupled chromatin assembly and is targeted to the replication fork via interactions with PCNA, which, if disrupted, leads to epigenetic defects. In Saccharomyces cerevisiae, when the silent mating-type locus HMR contains point mutations within the E silencer, Sir protein association and silencing is lost. However, mutation of CDC7, encoding an S-phase-specific kinase, or subunits of the H4 K16-specific acetyltransferase complex SAS-I, restore silencing to this crippled HMR, HMRae**. Here, we observed that loss of Cac1Δ, the largest subunit of CAF-1, also restores silencing at HMRae**, and silencing in both cac1D and cdc7 mutants is suppressed by overexpression of SAS2. We demonstrate Cdc7p and Cac1p interact in vivo in S phase, but not in G1, consistent with observed cell cycle-dependent phosphorylation of Cac1p, and hypoacetylation of chromatin at H4 K16 in both cdc7 and cac1Δ mutants. Moreover, silencing at HMRae** is restored in cells expressing cac1p mutants lacking Cdc7p phosphorylation sites. We also discovered that cac1Δ and cdc7- 90 synthetically interact negatively in the presence of DNA damage, but that Cdc7p phosphorylation sites on Cac1p are not required for responses to DNA damage. Combined, our results support a model in which Cdc7p regulates replication-coupled histone modification via a CAC1-dependent mechanism involving H4 K16ac deposition, and thereby silencing, while CAF-1-dependent replicationand repair-coupled chromatin assembly per se are functional in the absence of phosphorylation of Cdc7p consensus sites on CAF-1.

Original languageEnglish (US)
Pages (from-to)1219-1237
Number of pages19
JournalGenetics
Volume211
Issue number4
DOIs
StatePublished - Apr 2019

Fingerprint

Chromatin Assembly Factor-1
Gene Silencing
Acetylation
Saccharomyces cerevisiae
Phosphorylation
Chromatin Assembly and Disassembly
S Phase
DNA Damage
Histone Code
Histone Chaperones
Acetyltransferases
Proliferating Cell Nuclear Antigen
Point Mutation
Epigenomics
Chromatin
Cell Cycle
Phosphotransferases
Mutation
Proteins

Keywords

  • CAC1
  • CAF-1
  • CDC7
  • Histone H4 K16
  • SAS2
  • Silencing

ASJC Scopus subject areas

  • Genetics

Cite this

Modulation of gene silencing by Cdc7p via H4 K16 acetylation and phosphorylation of chromatin assembly factor CAF-1 in saccharomyces cerevisiae. / Young, Tiffany J.; Cui, Yi; Irudayaraj, Joseph; Kirchmaier, Ann L.

In: Genetics, Vol. 211, No. 4, 04.2019, p. 1219-1237.

Research output: Contribution to journalArticle

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AB - CAF-1 is an evolutionarily conserved H3/H4 histone chaperone that plays a key role in replication-coupled chromatin assembly and is targeted to the replication fork via interactions with PCNA, which, if disrupted, leads to epigenetic defects. In Saccharomyces cerevisiae, when the silent mating-type locus HMR contains point mutations within the E silencer, Sir protein association and silencing is lost. However, mutation of CDC7, encoding an S-phase-specific kinase, or subunits of the H4 K16-specific acetyltransferase complex SAS-I, restore silencing to this crippled HMR, HMRae**. Here, we observed that loss of Cac1Δ, the largest subunit of CAF-1, also restores silencing at HMRae**, and silencing in both cac1D and cdc7 mutants is suppressed by overexpression of SAS2. We demonstrate Cdc7p and Cac1p interact in vivo in S phase, but not in G1, consistent with observed cell cycle-dependent phosphorylation of Cac1p, and hypoacetylation of chromatin at H4 K16 in both cdc7 and cac1Δ mutants. Moreover, silencing at HMRae** is restored in cells expressing cac1p mutants lacking Cdc7p phosphorylation sites. We also discovered that cac1Δ and cdc7- 90 synthetically interact negatively in the presence of DNA damage, but that Cdc7p phosphorylation sites on Cac1p are not required for responses to DNA damage. Combined, our results support a model in which Cdc7p regulates replication-coupled histone modification via a CAC1-dependent mechanism involving H4 K16ac deposition, and thereby silencing, while CAF-1-dependent replicationand repair-coupled chromatin assembly per se are functional in the absence of phosphorylation of Cdc7p consensus sites on CAF-1.

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