Modified hybridoma methodology: Antigen-directed chemically mediated cell fusion

David M Kranz, James N. Herron, Patricia A. Billing, Edward W. Voss

Research output: Contribution to journalArticle

Abstract

The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.

Original languageEnglish (US)
Pages (from-to)639-651
Number of pages13
JournalImmunological Investigations
Volume9
Issue number7
DOIs
StatePublished - Jan 1 1980

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Cell Fusion
Hybridomas
Antigens
Ligands
Population

ASJC Scopus subject areas

  • Immunology

Cite this

Modified hybridoma methodology : Antigen-directed chemically mediated cell fusion. / Kranz, David M; Herron, James N.; Billing, Patricia A.; Voss, Edward W.

In: Immunological Investigations, Vol. 9, No. 7, 01.01.1980, p. 639-651.

Research output: Contribution to journalArticle

Kranz, David M ; Herron, James N. ; Billing, Patricia A. ; Voss, Edward W. / Modified hybridoma methodology : Antigen-directed chemically mediated cell fusion. In: Immunological Investigations. 1980 ; Vol. 9, No. 7. pp. 639-651.
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