TY - JOUR
T1 - Modified hybridoma methodology
T2 - Antigen-directed chemically mediated cell fusion
AU - Kranz, David M.
AU - Herron, James N.
AU - Billing, Patricia A.
AU - Voss, Edward W.
PY - 1980
Y1 - 1980
N2 - The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.
AB - The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.
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U2 - 10.3109/08820138009066015
DO - 10.3109/08820138009066015
M3 - Article
C2 - 7439926
AN - SCOPUS:84907107851
SN - 0882-0139
VL - 9
SP - 639
EP - 651
JO - Immunological Investigations
JF - Immunological Investigations
IS - 7
ER -