Modified hybridoma methodology: Antigen-directed chemically mediated cell fusion

David M Kranz, James N. Herron, Patricia A. Billing, Edward W. Voss

Research output: Contribution to journalArticle

Abstract

The concept of antigen-directed cell fusions to increase the yield of hybridomas was investigated. To facilitate cell-cell contact, antigen conjugated cells were used in cell fusion studies. Specifically, fluorescyl conjugated murine myeloma cells (Sp 2/0-Ag14) incubated with murine immune (anti-fluorescyl) splenocytes formed aggregates containing fluorescent and non-fluorescent cells. Fusion of these populations with polyethylene glycol resulted in a greater number of anti-fluorescyl hybridomas relative to normal fusions under non-antigen directed condition. Ligand binding data indicated that despite the multicellular aggregates the hybridomas resulting from chemically mediated fusions produced only one monoclonal Ig.

Original languageEnglish (US)
Pages (from-to)639-651
Number of pages13
JournalImmunological Investigations
Volume9
Issue number7
DOIs
StatePublished - Jan 1 1980

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ASJC Scopus subject areas

  • Immunology

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