Modified assay for determination of hydroxyproline in a tissue hydrolyzate

C. A. Edwards, W. D. O'Brien

Research output: Contribution to journalArticlepeer-review


A modified assay for the determination of hydroxyproline in tissue is presented. The modifications greatly reduce the time required for analysis of excised tissue as first introduced by Stegemann and Stalder [1]. These modifications include a change in the technique for tissue hydrolysis and a change in the preparation of the hydroxyproline oxidizing agent. The analysis utilizes the standard addition technique, eliminating the need for correction of matrix effects between the specimen and standard. This paper attempts to give a complete detailed description of the assay such that the procedure may be repeated without requiring additional reference material.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalClinica Chimica Acta
Issue number2
StatePublished - Jun 10 1980

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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