Modification of a mammalian cell protein in the presence of [ 32P-adenylate]NAD: Evidence for ADP ribosylation activity associated with Helicobacter pylori

Carlos W. Nossa; Steven R. Blanke

doi:10.1128/IAI.74.5.3071-3076.2006

Modification of a mammalian cell protein in the presence of [ ³²P-adenylate]NAD: Evidence for ADP ribosylation activity associated with Helicobacter pylori

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Abstract

Culture filtrates from Helicobacter pylori promote the transfer of the radiolabel from [³²P-adenylate]NAD to one or more heat-labile factors within extracts prepared from several mammalian cell lines, with the predominate radiolabeled species exhibiting an apparent molecular mass of greater than 130 kDa. Our results suggest that several H. pylori strains release a factor that ADP-ribosylates a mammalian target protein.

Original language	English (US)
Pages (from-to)	3071-3076
Number of pages	6
Journal	Infection and immunity
Volume	74
Issue number	5
DOIs	https://doi.org/10.1128/IAI.74.5.3071-3076.2006
State	Published - May 2006

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/IAI.74.5.3071-3076.2006

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